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首页> 外文学位 >STUDIES ON EIKENELLA CORRODENS: A GRAM-NEGATIVE BACTERIUM OF PERIODONTOPATHIC POTENTIAL: 1. ULTRASTRUCTURE AND MORPHOLOGY; 2. NUTRITION AND GROWTH; 3. OUTER MEMBRANE CHARACTERIZATION; 4. BIOLOGICAL ANALYSIS OF OUTER MEMBRANE COMPONENTS.
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STUDIES ON EIKENELLA CORRODENS: A GRAM-NEGATIVE BACTERIUM OF PERIODONTOPATHIC POTENTIAL: 1. ULTRASTRUCTURE AND MORPHOLOGY; 2. NUTRITION AND GROWTH; 3. OUTER MEMBRANE CHARACTERIZATION; 4. BIOLOGICAL ANALYSIS OF OUTER MEMBRANE COMPONENTS.

机译：埃肯氏菌的研究：牙周病潜力的革兰氏阴性细菌：1.超微结构和形态； 2.营养和增长； 3.外膜特性； 4.外膜成分的生物学分析。

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摘要

The ultrastructure, growth characteristics, outer membrane biochemistry, and biological activities of outer membrane components of the potential periodontopathogen, Eikenella corrodens, were studied. Electron microscopic observation of the type strain, ATCC 23834, and two periodontal disease isolates showed a typical Gram-negative cell envelope structure. Ruthenium red staining, alcian blue staining, heavy metal shadow-casting, and scanning electron microscopy revealed the presence of an extracellular slime layer.; The development of a liquid medium (containing salts, KNO(,3), hemin, and yeast extract) capable of supporting good growth of E. corrodens strains, allowed the study of parameters of Eikenella growth. Growth was found to be optimal in either a 10% CO(,2) + air or 100% air atmosphere while growth in an anaerobic atmosphere was less than optimal. Nutritionally, E. corrodens strain 23834 appears to require nitrate and one or more factors present in yeast extract for growth.; The purified outer membrane fractions (OMFs) were predominantly protein (69%) with 10% lipid. SDS-PAGE analysis revealed one major peptide of 33,500 dal in strain 23834 and 37,500 dal in strain 470 OMFs. The lipopolysaccharide (LPS) was found to contain lipid, carbohydrate, no hydroxy fatty acids, and only trace amounts of heptose and 2-keto-3-deoxyoctonate.; Despite a nonclassical LPS structure, the Eikenella LPS retained classical LPS biological activities, including mitogenicity, limulus lysate clotting, and bone resorption. The OMFs were also active in these assays. It thus appears that E. corrodens is well "equipped" to function as a major factor in periodontal tissue necrosis.
机译：研究了潜在牙周病原体艾肯氏菌的超微结构，生长特性，外膜生物化学和外膜成分的生物活性。对菌株ATCC 23834和两个牙周病分离株的电子显微镜观察显示出典型的革兰氏阴性细胞包膜结构。钌红色染色，阿尔辛蓝色染色，重金属阴影投射和扫描电子显微镜显示存在细胞外粘液层。能够支持E. corrodens菌株良好生长的液体培养基（包含盐，KNO（，3），血红素和酵母提取物）的发展，使得对Eikenella生长参数的研究成为可能。发现在10％CO（，2）+空气或100％空气气氛中生长最佳，而在厌氧气氛中生长则不理想。从营养上讲，腐蚀的大肠杆菌菌株23834似乎需要硝酸盐和酵母提取物中存在的一种或多种因子才能生长。纯化的外膜部分（OMF）主要是蛋白质（69％），脂质含量为10％。 SDS-PAGE分析揭示了一种主要肽，其在菌株23834中为33,500 dal，在菌株470 OMF中为37,500 dal。发现脂多糖（LPS）含有脂质，碳水化合物，不含羟基脂肪酸，并且仅含有痕量的庚糖和2-酮-3-脱氧辛酸酯。尽管LLP具有非经典的LPS结构，但Eikenella LPS仍保留了经典的LPS生物活性，包括有丝分裂性，溶解物凝结和骨吸收。 OMF在这些测定中也有活性。因此看来，腐蚀后的大肠杆菌被很好地“装备”为牙周组织坏死的主要因素。

著录项

  • 作者

    PROGULSKE, ANN.;

  • 作者单位

    University of Massachusetts Amherst.;

  • 授予单位 University of Massachusetts Amherst.;
  • 学科 Biology Microbiology.
  • 学位 Ph.D.
  • 年度 1983
  • 页码 225 p.
  • 总页数 225
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 微生物学;
  • 关键词

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