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首页> 外文学位 >Portulaca Oleracea Extract Can Inhibit Nodule Formation of Colon Cancer Stem Cells by Regulating Gene Expression of Notch Signal Transduction Pathway
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Portulaca Oleracea Extract Can Inhibit Nodule Formation of Colon Cancer Stem Cells by Regulating Gene Expression of Notch Signal Transduction Pathway

机译：马齿ula提取物可通过调节Notch信号转导通路的基因表达抑制结肠癌干细胞的结节形成

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摘要

Purpose: The main aim of this study was to investigate the effects of Portulaca oleracea extract on tumor formation in colon cancer stem cells and chemotherapy sensitivity. In addition, this study analyzed the genetic changes within the Notch signal transduction pathway associated with the effects of the extracts.;Methods: Serum-free cultures of colon cancer cells (HT-29) and HT-29 stem cells were treated with the chemotherapeutic drug 5-FU to assess sensitivity. Injections of the stem cells were also given to BALB/c mice to confirm tumor growth and characteristics. In addition, the effect of different concentrations of P. oleracea extract was tested on the growth of HT-29 and HT-29 stem cells, which was determined by the MTT method. The effects of P. oleracea extract on the expression of beta-catenin, Notch1, and Notch2 in HT-29 cells were detected by RT-PCR and Western blotting.;Results: The tumor volume of HT29 cells was two times larger than that of HT29 stem cells. Treatment with P. oleracea extracts inhibited the proliferation of both HT-29 and HT-29 stem cells in doses from 0.07 - 2.25 mug/mL. Apoptosis of HT-29 and HT-29 stem cells, as assessed by flow cytometry, was enhanced by the addition of P. oleracea extract. Finally, treatment with P. oleracea extracts significantly down-regulated the expression of the Notch1 and beta-catenin genes in both cell types.;Conclusion: This study shows that P. oleracea extract inhibits the growth of colon cancer stem cells in a dose-dependent manner. Furthermore, it inhibits the expression of the Notch1 and beta-catenin genes. Taken together, this suggests that it may elicit its effects through regulatory and target genes that mediate the Notch signal transduction pathway.
机译：目的：本研究的主要目的是研究马齿Port提取物对结肠癌干细胞肿瘤形成和化疗敏感性的影响。此外，本研究还分析了Notch信号转导途径中与提取物作用有关的遗传变化。方法：对结肠癌细胞（HT-29）和HT-29干细胞的无血清培养物进行化学治疗药物5-FU评估敏感性。还向BALB / c小鼠注射干细胞，以确认肿瘤的生长和特征。此外，测试了不同浓度的油茶提取物对HT-29和HT-29干细胞生长的影响，这是通过MTT方法确定的。 RT-PCR和Western blotting检测油桐提取物对HT-29细胞中β-catenin，Notch1和Notch2表达的影响；结果：HT29细胞的肿瘤体积是HT29细胞的两倍。 HT29干细胞。 P. oleracea提取物处理可抑制HT-29和HT-29干细胞的增殖，剂量范围为0.07-2.25 mug / mL。如通过流式细胞术评估的，HT-29和HT-29干细胞的凋亡通过添加甘蓝假单胞菌提取物得到增强。最后，用桔梗提取物处理可显着下调两种细胞类型中Notch1和β-catenin基因的表达。结论：这项研究表明，桔梗提取物在一定剂量下抑制结肠癌干细胞的生长。依赖方式。此外，它抑制Notch1和β-catenin基因的表达。两者合计，这表明它可能通过介导Notch信号转导途径的调节基因和靶基因引起其效应。

著录项

  • 作者

    Chen, Li.;

  • 作者单位

    Michigan Technological University.;

  • 授予单位 Michigan Technological University.;
  • 学科 Biochemistry.;Molecular biology.
  • 学位 Ph.D.
  • 年度 2016
  • 页码 111 p.
  • 总页数 111
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

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