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首页> 外文学位 >X-RAY CRYSTALLOGRAPHIC AND SPECTROSCOPIC INVESTIGATIONS ON A PHYCOBILIPROTEIN: C-PHYCOCYANIN FROM THE CYANOPHYTE AGMENELLUM QUADRUPLICATUM.
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X-RAY CRYSTALLOGRAPHIC AND SPECTROSCOPIC INVESTIGATIONS ON A PHYCOBILIPROTEIN: C-PHYCOCYANIN FROM THE CYANOPHYTE AGMENELLUM QUADRUPLICATUM.

机译：藻生物蛋白的X射线晶体学和光谱学研究：古生​​蓝藻中的C-藻蓝蛋白。

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摘要

C-phycocyanin (CPC), an accessory photosynthetic pigment from the cyanobacterium Agmenellum quadruplicatum was examined using X-ray crystallographic techniques, electron microscopy, and absorbance and fluorescence spectroscopy. Crystals of CPC belong to space group P321 with a = b = 184.5 (ANGSTROM) and c = 60.5 (ANGSTROM). Three dimensional data were collected by the oscillation method. Three isomorphous derivatives were found and conditions refined.;Modeling studies show the crystallographic unit cell to be made up of three ((alpha)(beta))(,6) hexamers centered on the crystallographic tryads and being rotated 30(DEGREES) relative to one another. The hexamers themselves are made up to two ((alpha)(beta))(,3) trimers joined face to face and rotated 60(DEGREES) with respect to one another.;Rotation function studies demonstrated the presence of a molecular six-fold axis of symmetry parallel to the crystallographic tryads as well as molecular dyads intersecting the crystallographic two-fold rotation axes.;The visible absorption of solution CPC and a suspension of CPC crystals showed little difference while crystals of CPC and BPE (B-phycoerythrin, another phycobiliprotein, from the red alga Porphyridium cruentum) fluorescence was red shifted 50-60 nm relative to their solution forms. Biological implications are discussed.;Electron microscopy of crystals sectioned perpendicular to the crystallographic c-axis shows a hexagonal closest packing arrangement of donut-shaped particles about 100 (ANGSTROM) in diameter.;CPC and BPE crystals also demonstrated shortened fluorescence lifetimes relative to their solution counterparts.
机译：使用X射线晶体学技术，电子显微镜以及吸光度和荧光光谱法检查了C-藻蓝蛋白（CPC），它是四倍体蓝藻农杆菌的辅助光合作用色素。 CPC晶体属于a = b = 184.5（ANGSTROM）和c = 60.5（ANGSTROM）的空间群P321。通过振动法收集了三维数据。找到了三个同构的衍生物并完善了条件。;建模研究表明，晶体学晶胞由三个（α）（β））（，6）六聚体组成，这些六聚体以晶体试棒为中心，相对于晶体旋转30°（DEGREES）。另一个。六聚体本身由两个面对面连接并彼此相对旋转60（DEGREES）的（α）（β）（，3）三聚体组成。旋转功能研究表明存在六倍分子平行于晶体三臂轴的对称轴以及与晶体双轴旋转轴相交的分子二倍体。;溶液CPC的可见吸收和CPC晶体的悬浮液几乎没有差异，而CPC和BPE的晶体（B-藻红蛋白，另一种来自红藻蓝藻（Porphyridium cruentum）的藻胆蛋白荧光相对于其溶液形式红移了50-60 nm。讨论了生物学意义。;垂直于晶体学c轴截面的晶体的电子显微镜显示直径约100（ANGSTROM）的甜甜圈形颗粒的六边形最紧密堆积排列;; CPC和BPE晶体相对于它们的荧光寿命也缩短了解决方案同行。

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  • 作者

    PRIESTLE, JOHN PETER, JR.;

  • 作者单位

    The University of Texas at Austin.;

  • 授予单位 The University of Texas at Austin.;
  • 学科 Biochemistry.
  • 学位 Ph.D.
  • 年度 1982
  • 页码 251 p.
  • 总页数 251
  • 原文格式 PDF
  • 正文语种 eng
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