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COMPARATIVE NUCLEIC ACID STUDIES ON CITRUS STUBBORN AND CORN STUNT SPIROPLASMAS.

机译:柑橘胚芽和玉米矮生子螺旋体的比较核酸研究。

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摘要

The ribosomal ribonucleic acids (rRNAs) of Spiroplasma citri and corn stunt spiroplasma (CSS) were compared with rRNAs of Acholeplasma laidlawii, Mycoplasma hominis, Bacillus subtilis, and Escherichia coli by polyacrylamide gel electrophoresis. In nondenaturing gels, the 23s rRNA of spiroplasmas and B. subtilis migrated faster than the 23s rRNA of A. laidlawii and E. coli, but the 16s rRNAs comigrated. In denaturing gels, the 16s rRNAs behaved similarly but the 23s rRNAs of spiroplasmas and A. laidlawii migrated faster than their bacterial counterpart. The 5s rRNA of S. citri and CSS was 35000 daltons, intermediate in size between 5s rRNAs of A. laidlawii (36500 daltons) and M. hominis (32200 daltons). The 5s rRNA of A. laidlawii was, in turn, smaller than the bacterial 5s rRNAs. It is concluded that mycoplasmas have the smallest 5s rRNA reported.; Deoxyribonucleic acid hybridization studies were performed to assess the degree of genetic relatedness between CSS and S. citri. At 56(DEGREES)C, the degree of homology between CSS and S. citri was about 68% (63 to 75%). The level of homology among four S. citri isolates tested ranged from 93 to 100%. The protein patterns of S. citri isolates were identical on polyacrylamide gels but partial similarity was observed between CSS and S. citri. It is suggested that CSS and S. citri belong to the same species but to separate subspecies or varieties.
机译:通过聚丙烯酰胺凝胶电泳比较了柠檬螺旋体和玉米特技螺旋体(CSS)的核糖体核糖核酸(rRNA),以及无花小圆虫,人型支原体,枯草芽孢杆菌和大肠杆菌的rRNA。在非变性凝胶中,螺旋体和枯草芽孢杆菌的23s rRNA迁移的速度比莱德瓦利曲霉和大肠杆菌的23s rRNA迁移快,但是16s rRNA迁移了。在变性凝胶中,16s rRNA的行为相似,但螺旋体和裸露曲霉的23s rRNA的迁移速度要快于细菌。柠檬葡萄球菌和CSS的5s rRNA为35000道尔顿,介于A. laylawii(36500道尔顿)和M. hominis(32200道尔顿)的5s rRNA之间。反而,A.laidlawii的5s rRNA小于细菌的5s rRNA。结论是支原体报告的最小5s rRNA。进行了脱氧核糖核酸杂交研究,以评估CSS和柠檬葡萄球菌之间的遗传相关程度。在56℃时,CSS和柠檬葡萄球菌之间的同源性约为68%(63%至75%)。四个测试的柠檬酸链球菌分离株之间的同源性水平为93%至100%。在聚丙烯酰胺凝胶上,柠檬葡萄球菌分离物的蛋白质模式相同,但在CSS和柠檬葡萄球菌之间观察到部分相似。建议CSS和柠檬柑属于同一物种,但属于不同的亚种或变种。

著录项

  • 作者

    RAHIMIAN, HESHMATOLLAH.;

  • 作者单位

    University of California, Riverside.;

  • 授予单位 University of California, Riverside.;
  • 学科 Agriculture Plant Pathology.
  • 学位 Ph.D.
  • 年度 1980
  • 页码 52 p.
  • 总页数 52
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 植物病理学;
  • 关键词

  • 入库时间 2022-08-17 11:51:39

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