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INVESTIGATIONS INTO THE NATURE OF SPIROPLASMA-LIKE BODIES ASSOCIATED WITH CERATOCYSTIS ULMI.

机译：对与乌拉圭巨噬细胞相关的类螺旋体尸体性质的调查。

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摘要

Three of six N.Y. Ceratocystis ulmi (Buism.) C. Moreau isolates examined using electron microscopy contained spiroplasma-like bodies. The fact that cultures of isolates which were treated with 30 ug/ml tetracycline hydrochloride lacked spiroplasma-like bodies, suggests that these bodies may be prokaryotic structures. Spiroplasma-like bodies were found to occur in C. ulmi isolates which appeared to be weakly to moderately virulent as determined from trunk inoculations of 10-year-old American elms (Ulmus americana L.). No relationship was found between the occurrence of spiroplasma-like bodies and growth form and mating type of C. ulmi. Cultural characteristic data suggests that tetracycline treatment of isolates which contain spiroplasma-like bodies may induce different physiological effects than treatment of isolates which do not contain them. Of seven C. ulmi isolates received from the University of Maine, three of four non-aggressive type isolates produced particles on a synthetic agar medium which resembled those produced by three N.Y. isolates known to contain spiroplasma-like bodies within their hyphae. Three aggressive type isolates from the University of Maine did not produce such particles. This suggests that non-aggressive type isolates may be more likely to contain spiroplasma-like bodies than aggressive type ones. DNA fluorescent Hoechst 33258 stain could not be successfully utilized to discriminate spiroplasma-like bodies from nuclei, mitochondria, and other fluorescent structures within hyphal cells.
机译：使用电子显微镜检查的六个N.Y. Ceratocystis ulmi（Buism。）C. Moreau分离物中的三个含有螺旋体样体。用30 ug / ml盐酸四环素处理过的分离株的培养物中缺乏螺旋体状的小体，这表明这些小体可能是原核结构。发现螺旋体状体出现在乌氏梭状芽胞杆菌分离株中，从10岁美洲榆（Ulmus americana L.）的树干接种确定，其似乎弱至中等毒性。螺旋体状体的发生与生长形态和交配类型之间没有关系。文化特征数据表明，对含有螺旋体样体的分离株进行四环素处理可能会产生与不含有螺旋体样分离株的分离株不同的生理效应。在从缅因州大学获得的7个ul。C. ulmi分离株中，四个非侵略性分离株中的三个在合成琼脂培养基上产生了颗粒，类似于在其菌丝中含有螺旋体状体的三个N.Y.分离株产生的颗粒。来自缅因大学的三种侵略性分离株未产生此类颗粒。这表明非侵略性分离株比侵袭性分离株更可能含有螺旋体样体。 DNA荧光Hoechst 33258染色不能成功地用于从菌丝细胞内的细胞核，线粒体和其他荧光结构中区分螺旋体状体。

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  • 作者

    GOWEN, PATRICIA ELIZABETH.;

  • 作者单位

    State University of New York College of Environmental Science and Forestry.;

  • 授予单位 State University of New York College of Environmental Science and Forestry.;
  • 学科 Agriculture Plant Pathology.
  • 学位 Ph.D.
  • 年度 1980
  • 页码 57 p.
  • 总页数 57
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 植物病理学;
  • 关键词

  • 入库时间 2022-08-17 11:51:40

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