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Microfluidic Device for the Evaluation of Biofilm Removal under Shear Stress.

机译：用于评估剪切应力下生物膜去除的微流体装置。

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摘要

Biofilms are a relevant problem in the medical field and many other industries. Biofilms can lead to proliferation of pathogens, loss of life, equipment failure, and loss in productivity. Biofilms also have increased resistance to antibiotics and are harder to remove than planktonic bacteria. Motile bacteria behave differently than the immotile bacteria that compose biofilms, and so studies that focus specifically on biofilms are necessary.;The experiments consisted of growing biofilms Pseudomonas aeruginosa, subjecting them to stress in the presence of a removal agent, and capturing images of the biofilms. A microfluidic device allowed for the use of existing microscope setups to image the biofilms, which allows for broad application of the device. The microfluidic device also mimicked the scale at which bacteria operate. Pressure was applied at increasing magnitude to find the threshold at which the biofilm began to be removed. The images were then analyzed to determine the amount of biofilm removal that occurred.;Polydimethylsiloxane (PDMS) microfluidic devices were used to evaluate treatment methods in conjunction with shear stress. The experiments that were conducted show the differences in the effect of shear stress with two removal agents on a biofilm. The pressures at which phosphate buffered saline (PBS) and sodium dodecyl sulfate (SDS) removed biofilms were statistically different. SDS required a lower pressure threshold to remove the biofilm than PBS. The percentage of biofilm that was removed was also statistically different between the PBS and SDS. At their respective removal pressures and in the same time span, SDS removed more of the biofilm than PBS.;The biofilms were characterized using confocal imaging, which creates a 3D reconstruction of the biofilm. The biofilms were 2.5 microm thick and had one to two layers of bacteria. The biofilms in the microfluidic device have an average radius of 28.3+/-11.5 microm after overnight growth.;The shear stress applied to the biofilm was modeled using COMSOL MultiphysicsRTM software. The model includes a profile of the shear stress in the fluid as a function of position in the working fluid. Pressure-driven flow rates and biofilm size were varied in the model. The location of the maximum shear correlates with the portion of the biofilm that faces and extends into the fluid flow. The model shows, as expected, that increasing biofilm size decreases shear stress and increasing flow rate increases the shear stress.;The described device has been used to successfully to compare chemical treatment methods while applying a shear stress to the biofilms.
机译：生物膜是医学领域和许多其他行业中的一个相关问题。生物膜可能导致病原体扩散，生命损失，设备故障以及生产力下降。生物膜对抗生素的抵抗力也增强，比浮游细菌更难去除。运动细菌的行为与构成生物膜的运动细菌的行为不同，因此有必要进行专门针对生物膜的研究。实验包括生长生物膜的铜绿假单胞菌，在有去除剂的情况下使其承受压力，并捕获生物膜的图像。生物膜。微流体装置允许使用现有的显微镜设置来对生物膜成像，这允许该装置的广泛应用。微流体装置也模仿细菌运转的规模。以增加的幅度施加压力以找到开始去除生物膜的阈值。然后对图像进行分析，以确定发生的生物膜去除量。聚二甲基硅氧烷（PDMS）微流体装置用于结合剪切应力评估处理方法。进行的实验表明，两种去除剂对生物膜的剪切应力影响不同。磷酸盐缓冲盐水（PBS）和十二烷基硫酸钠（SDS）去除生物膜的压力在统计学上是不同的。与PBS相比，SDS需要较低的压力阈值才能去除生物膜。在PBS和SDS之间，被去除的生物膜的百分比在统计学上也不同。在各自的去除压力和相同的时间跨度下，SDS去除的生物膜比PBS还要多。;使用共聚焦成像对生物膜进行表征，从而对生物膜进行3D重建。生物膜的厚度为2.5微米，并具有一到两层细菌。过夜生长后，微流体装置中的生物膜的平均半径为28.3 +/- 11.5微米。;使用COMSOL MultiphysicsRTM软件对施加到生物膜上的剪切应力进行建模。该模型包括流体中的剪切应力随工作流体中位置的变化曲线。在模型中，压力驱动的流速和生物膜的大小有所不同。最大剪切力的位置与生物膜面对并延伸到流体流中的部分相关。该模型表明，如预期的那样，增加生物膜的尺寸会减小剪切应力，而增加流速会增加剪应力。所描述的设备已成功用于比较化学处理方法，同时对生物膜施加了剪应力。

著录项

  • 作者

    Huo, Bowen.;

  • 作者单位

    Northeastern University.;

  • 授予单位 Northeastern University.;
  • 学科 Chemical engineering.;Microbiology.
  • 学位 M.S.
  • 年度 2015
  • 页码 47 p.
  • 总页数 47
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

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