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首页> 外文学位 >Activity of mannanase in relation to mixed micelle formation of linear alkyl benzene sulfonate with linear ethoxylated alcohol.
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Activity of mannanase in relation to mixed micelle formation of linear alkyl benzene sulfonate with linear ethoxylated alcohol.

机译：甘露聚糖酶的活性与线性烷基苯磺酸盐与线性乙氧基化醇混合胶束形成有关。

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摘要

Anionic surfactants, such as linear alkyl benzene sulfonate (LAS) can inhibit enzyme activity in detergent formulations. The addition of a nonionic surfactant, such as an alcohol ethoxylate (AEO), can prevent the loss of enzyme activity caused by the LAS. This research was performed to assess if these general observations hold true in the specific case of MannawayRTM mannanase detergent enzyme in the presence of LAS and Neodol 25-7RTM an ethoxylated alcohol. This study evaluates the hypothesis that free LAS, i.e. LAS not participating in an assembled micelle, causes the decreased enzyme activity and that presence of AEO catalyzes the formation of mixed LAS:AEO micelles, reducing the concentration of free LAS in solution and its adverse effect on mannanase activity. The effect of the formation of LAS:AEO mixed micelles on the enzymatic activity of mannanase and the concentration of free LAS in solutions were studied with a combination of tensiometric, potentiometric and enzymatic assays to provide experimental evidence of the link between mixed surfactant micelle formation, reduced free LAS concentration and mannanase activity. Results indicate that the formation of mixed micelles does cause a decrease of free LAS which allows for normal mannanase activity in the formulated mixture. This study proposes an equilibrium based model to calculate the concentration of free LAS activity in a mixed surfactant LAS:AEO solution and to predict the extent of an adverse effect of free LAS to mannanase activity.
机译：阴离子表面活性剂，例如线性烷基苯磺酸盐（LAS）可以抑制洗涤剂配方中的酶活性。加入非离子表面活性剂，例如乙醇乙氧基化物（AEO），可以防止LAS引起的酶活性损失。进行这项研究的目的是评估在LAS和Neodol 25-7RTM为乙氧基化醇的情况下，对于MannawayRTM甘露聚糖酶去污剂酶的具体情况，这些一般观察是否成立。这项研究评估以下假设：游离LAS（即未参与组装的胶束的LAS）导致酶活性降低，并且AEO的存在催化混合LAS：AEO胶束的形成，从而降低了溶液中游离LAS的浓度及其不利影响甘露聚糖酶活性。结合张力，电位和酶法试验研究了LAS：AEO混合胶束的形成对甘露聚糖酶酶活性和溶液中游离LAS浓度的影响，以提供混合表面活性剂胶束形成之间联系的实验证据，降低游离LAS浓度和甘露聚糖酶活性。结果表明，混合胶束的形成确实会导致游离LAS降低，从而使配制的混合物中的甘露聚糖酶活性正常。这项研究提出了一个基于平衡的模型，以计算混合表面活性剂LAS：AEO溶液中游离LAS活性的浓度，并预测游离LAS对甘露聚糖酶活性的不利影响程度。

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  • 作者

    Seyfert, Jeannine D.;

  • 作者单位

    University of Massachusetts Boston.;

  • 授予单位 University of Massachusetts Boston.;
  • 学科 Chemistry.
  • 学位 M.S.
  • 年度 2015
  • 页码 82 p.
  • 总页数 82
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

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