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首页> 外文学位 >Design and Optimization of a System for Automated Culture Medium Exchange in a Petri Dish.
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Design and Optimization of a System for Automated Culture Medium Exchange in a Petri Dish.

机译：培养皿中自动培养基交换系统的设计和优化。

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摘要

To culture a sample, one method is to put an experimental specimen into a culture medium and to wait for it to grow. Some samples must be cultured for specific requirements in biological experiments. Scientists need to change the culture medium during some experiments. There are some automated pipetting systems and perfusion systems that can be used to exchange culture medium. However, all current products have some limitations. At present, there is no appropriate method or device to automatically exchange culture medium in a petri dish for some specific samples, such as tadpoles.;The major goal of this research was to design and demonstrate a device that can automatically exchange the culture medium in a petri dish. The automatic exchange device would have following advantages: flexible exchange settings, easy operation, no risk of dehydration, high exchange efficiency and low cost. This device would be a cost effective solution for automating tedious routine lab work, and may greatly promote new developments in biology.;The device designed in this thesis mainly included two parts: an emptying system and a filling system. A sample device was manufactured to examine the filling system design and test the efficiency of the emptying system using a petri dish as the culture container. Two basic models and a series of adjustments were investigated for use in the emptying system. In order to explore the most efficient model, finite element analysis was performed to simulate the fluid flow in the petri dish, and simulation results were compared to experimental data.
机译：为了培养样品，一种方法是将实验样品放入培养基中并等待其生长。为了生物学实验中的特定要求，必须对某些样品进行培养。科学家需要在一些实验中更换培养基。有一些自动移液系统和灌注系统可用于交换培养基。但是，当前所有产品都有一些局限性。目前，没有合适的方法或设备可以自动交换培养皿中某些特定样品（例如t）的培养基。该研究的主要目的是设计和演示一种可以自动交换培养皿中培养基的设备。培养皿。该自动交换装置具有以下优点：交换设置灵活，操作简便，无脱水风险，交换效率高，成本低。该装置将是自动化繁琐的常规实验室工作的经济有效的解决方案，并可能极大地促进生物学的新发展。本文设计的装置主要包括两部分：排空系统和填充系统。制造了一个样品装置，以检查填充系统的设计并使用培养皿作为培养容器来测试排空系统的效率。研究了用于排空系统的两个基本模型和一系列调整。为了探索最有效的模型，进行了有限元分析以模拟培养皿中的流体流动，并将模拟结果与实验数据进行比较。

著录项

  • 作者

    Yan, Luqi.;

  • 作者单位

    Tufts University.;

  • 授予单位 Tufts University.;
  • 学科 Mechanical engineering.
  • 学位 M.S.
  • 年度 2015
  • 页码 122 p.
  • 总页数 122
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

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