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首页> 外文学位 >Applying Molecular and Phenotypic Tools to Characterize Flesh Texture and Acidity Traits in the Arkansas Peach Breeding Program and Understanding the Crispy Texture in the Arkansas Blackberry Breeding Program.
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Applying Molecular and Phenotypic Tools to Characterize Flesh Texture and Acidity Traits in the Arkansas Peach Breeding Program and Understanding the Crispy Texture in the Arkansas Blackberry Breeding Program.

机译：在阿肯色州桃育种计划中应用分子和表型工具表征果肉质地和酸度特征，并在阿肯色黑莓育种计划中了解脆皮质地。

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摘要

Quantitative trait loci (QTL) utilizing a pedigree-based analysis (PBA) approach was performed in the peach breeding program for the first time. The pedigree consisted of seven-F1 populations, their parents, ancestors, and cultivars. Flesh firmness and titratable acidity (TA) were examined in 2011, 2012, and 2013. For TA, a consistent and strong QTL was identified on the proximal end of linkage group (LG) 5 of the peach genome. For flesh firmness, two QTLs were located on LG 4. The first QTL was located on the chromosomal region where the slow-melting flesh (SMF) DNA test was identified, and the second QTL was identified in the region of the endopolygalacturonase (endoPG) gene. However, the QTLs on LG 4 were not always consistent. These results indicate that PBA approach for QTL analysis can be applied with success in this mature and ongoing peach breeding program with the aim to find molecular markers associated with relevant quality traits, which is the first step to apply marker-assisted breeding (MAB). Also, DNA tests associated with TA and flesh firmness were analyzed with data taken in 2013 and 2014 on this pedigree and other seedlings, selections, and cultivars to predict acidity levels and flesh texture with the final goal to validate these DNA tests and apply MAB. These tests were able to predict correctly the expected acidity levels and flesh textures of the tested individuals.;Firmness and texture are critical traits in blackberry for breeders, growers, and consumers. Crispy and extremely firm fruits were characterized for two blackberry selections which had been observed to have low color reversion (a postharvest disorder). Firmness of these selections and its seedlings were tested in 2013 and 2014. Results indicated that crispy selections had superior firmness and a higher postharvest storage potential compared to cultivars and other selections in the program. These results are important, because firmness will be increased and color reversion will be reduced by use of this germplasm. This research also contributed to better understanding of the physical aspects of crispy and non-crispy genotypes, providing more information about this aspect of blackberries.
机译：首次在桃子育种程序中使用基于谱系的分析（PBA）方法进行了数量性状基因座（QTL）。家谱由七个F1人口，其父母，祖先和品种组成。在2011年，2012年和2013年检查了肉的硬度和可滴定酸度（TA）。对于TA，在桃基因组连锁群（LG）5的近端鉴定出一致且强的QTL。为了确保果肉的硬度，两个QTL位于LG 4上。第一个QTL位于鉴定了慢熔果肉（SMF）DNA测试的染色体区域，第二个QTL位于内聚半乳糖醛酸酶（endoPG）区域。基因。但是，LG 4上的QTL并不总是一致的。这些结果表明，用于QTL分析的PBA方法可以在此成熟且正在进行的桃子育种计划中成功应用，目的是寻找与相关质量性状相关的分子标记，这是应用标记辅助育种（MAB）的第一步。此外，利用2013年和2014年在该谱系和其他幼苗，选择和品种上获得的数据对与TA和果肉硬度相关的DNA测试进行了分析，以预测酸度水平和果肉质地，最终目的是验证这些DNA测试并应用MAB。这些测试能够正确预测被测个体的预期酸度水平和果肉质地。硬度和质地是黑莓育种者，种植者和消费者的关键特征。对于两个黑莓选择，其特征是酥脆和非常坚硬的水果，已发现它们具有较低的颜色还原（采后障碍）。在2013年和2014年对这些选择及其幼苗的牢固性进行了测试。结果表明，与该计划中的栽培品种和其他选择相比，脆皮选择具有更高的牢固性和更高的收获后储存潜力。这些结果很重要，因为通过使用这种种质将增加硬度并减少颜色还原。这项研究还有助于更好地了解脆性和非脆性基因型的物理方面，从而提供了有关黑莓这一方面的更多信息。

著录项

  • 作者

    Salgado Rojas, Alejandra.;

  • 作者单位

    University of Arkansas.;

  • 授予单位 University of Arkansas.;
  • 学科 Genetics.;Horticulture.
  • 学位 Ph.D.
  • 年度 2015
  • 页码 383 p.
  • 总页数 383
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

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