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Loop-Mediated Isothermal Amplification for the Mapping of Microbes: An Anti-Capitalist Approach

机译：环路介导的等温扩增微生物作图：一种反资本主义的方法。

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摘要

The field of microbial ecology is hindered by our general inability to map microbes on a geographical scale. This is in part due to technical limitations posed by current DNA detection reactions. This dissertation presents the application of Loop-Mediated Isothermal Amplification (LAMP) for the purpose of amplifying DNA from pollen grains; down to a single grain and with a resulting fluorescence signal to indicate the presence or absence of the particular DNA fragment in question. Three academic papers outlining this method comprise the bulk of the work and are bookended by a brief survey of microbial detection efforts to date and future applications of our method.;Beyond technical limitations there are social and political priorities embedded within the biological sciences that are implicit in our inability to map microbes. Therefore, any advance in the resolution of microbial mapping must also challenge these priorities. In place of the centralized research conducted by experts in universities and corporations, and ultimately for the purpose of capital accumulation, we propose a distributed and horizontally organized mapping network antagonistic to capitalist biology.;Pollen grains are our choice of microbe because they are the vector of reproduction for flowering plants and thus an important source of contamination of transgenic DNA from transgenic plants. The growing rejection of transgenic crops across the globe points to a strong desire to identify their location, utilizing microbial mapping methods, and eliminate their spread.;The technical advances presented here, alongside our social and political commitments, aim to assist in that effort.
机译：微生物生态学领域由于我们通常无法在地理规模上绘制微生物图而受到阻碍。这部分是由于当前DNA检测反应造成的技术限制。本文介绍了环介导的等温扩增（LAMP）技术在花粉粒中扩增DNA的应用。下降到单个颗粒，并产生荧光信号以指示是否存在所讨论的特定DNA片段。概述该方法的三篇学术论文构成了大部分工作，并通过对微生物检测迄今的努力和我们方法的未来应用的简要调查而被书记为书;除了技术上的局限性外，生物科学内含的社会和政治优先事项也隐含在其中我们无法绘制微生物图。因此，微生物标测分辨率的任何进步也必须挑战这些优先事项。代替大学和公司专家进行的集中研究，并最终出于资本积累的目的，我们提出了一个与资本主义生物学相对立的分布式，水平组织的映射网络。花粉谷物是我们选择的微生物，因为它们是媒介开花植物的生殖能力，因此是转基因植物中转基因DNA污染的重要来源。全球对转基因作物的排斥日益强烈，这表明人们强烈希望利用微生物作图方法来确定其位置，并消除其传播。此处提出的技术进步以及我们的社会和政治承诺，旨在协助这一努力。

著录项

  • 作者

    Tonak, Ali Bektas.;

  • 作者单位

    University of California, Berkeley.;

  • 授予单位 University of California, Berkeley.;
  • 学科 Ecology.;Microbiology.;Environmental science.
  • 学位 Ph.D.
  • 年度 2015
  • 页码 91 p.
  • 总页数 91
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

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