• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文学位 >Microfabricated Electrochemical Sensor for In Vivo Detection of Neurotransmitters and Metabolites.
【24h】

Microfabricated Electrochemical Sensor for In Vivo Detection of Neurotransmitters and Metabolites.

机译：体内检测神经递质和代谢物的超细电化学传感器。

获取原文
获取原文并翻译 | 示例
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

The electrochemical detection of neurotransmitters and metabolites in vivo has centered on fast-scan cyclic voltammetry (FSCV) due to its subsecond temporal resolution, sensitivity and chemical selectivity. FSCV at carbon fiber microelectrodes (CFMs) is a differential technique that can record phasic (second-to-second) changes in the concentration of electroactive analytes in a microenvironment, such as dopamine neurotransmission in the brain. However, in order to isolate current due to fluctuations in analyte concentration, i.e. to make phasic measurements, the subtraction of a large background current is required. This background current is generated by faradaic and non- Faradaic processes. Its volatility renders FSCV unable to determine absolute or slowly varying concentrations. However, there is still a need to readily determine the absolute or slowly changing concentrations of electroactive analytes in tissue. For example, basal concentrations of dopamine vary throughout the brain and can affect the dynamics of dopaminergic systems.;This research presents a microfabricated electrochemical sensor designed to improve on traditional FSCV at CFMs by adding the capability for absolute concentration measurements. The sensor design is small enough to integrate into an existing in vivo platform. The sensor is a collector-generator assembly comprised of carbon band electrodes spaced several microns apart. Absolute concentration sensing works by varying the potential of the generator electrode to manipulate local concentrations of electroactive species in the solution. These changes are measured at the adjacent collector electrode with FSCV and used to determine the absolute concentration in the solution. As a proof of concept, the sensor was used to determine absolute concentrations of ascorbic acid, an important reducing agent and metabolite. (Abstract shortened by ProQuest.).
机译：由于其亚秒级的时间分辨率，灵敏度和化学选择性，体内神经递质和代谢产物的电化学检测已集中在快速扫描循环伏安法（FSCV）上。碳纤维微电极（CFMs）上的FSCV是一种差分技术，可以记录微环境中电活性分析物浓度的相位变化（每秒变化），例如大脑中的多巴胺神经传递。然而，为了隔离由于分析物浓度的波动引起的电流，即进行相位测量，需要减去大的本底电流。该背景电流是由法拉第和非法拉第过程产生的。它的挥发性使FSCV无法确定绝对浓度或缓慢变化的浓度。然而，仍然需要容易地确定组织中电活性分析物的绝对或缓慢变化的浓度。例如，多巴胺的基础浓度在整个大脑中会发生变化，并且会影响多巴胺能系统的动力学。这项研究提出了一种微型电化学传感器，旨在通过增加绝对浓度测量的能力来改进CFM上的传统FSCV。传感器设计足够小，可以集成到现有的体内平台中。传感器是一个集电极-发生器组件，由几个微米间距的碳带电极组成。绝对浓度检测通过改变发生器电极的电势来控制溶液中电活性物质的局部浓度而起作用。这些变化是使用FSCV在相邻的集电极上测量的，并用于确定溶液中的绝对浓度。作为概念证明，该传感器用于确定抗坏血酸（一种重要的还原剂和代谢产物）的绝对浓度。 （摘要由ProQuest缩短。）。

著录项

  • 作者

    Dengler, Adam Kwok Hing.;

  • 作者单位

    North Carolina State University.;

  • 授予单位 North Carolina State University.;
  • 学科 Biomedical engineering.;Neurosciences.;Chemistry.
  • 学位 Ph.D.
  • 年度 2015
  • 页码 188 p.
  • 总页数 188
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号