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Development of a modified calcium-based composite ceramic bone graft material.

机译：改性钙基复合陶瓷骨移植材料的开发。

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摘要

Composites consisting of both calcium aluminum oxide and hydroxyapatite were tested for their applicability as bone replacement scaffolds. Implanted bone scaffolds may fail due to a variety of reasons, including mechanical and biological failure. Mechanical failure may occur as a result of dissimilar properties between the surrounding healthy bone and the scaffold. Biological failure can occur due to integration problems at the interface of the scaffold and the natural bone, or as a result of implant associated infections as a result of bacterial attachment and biofilm formation. Calcium aluminum oxide:hydroxyapatite composites were developed that address these three modes of implant failure through physical modification of the composition of the materials and chemical modification of the interface of the material.;The composites were evaluated for phase composition, elastic modulus, modulus of rupture, degradability, osteoblast attachment, percent viability and proliferation. Characterization was completed using powder x-ray diffraction (PXRD), a four point bending test, scanning electron microscopy (SEM), scanning electron microscopy-energy dispersive x-ray spectroscopy (SEM-EDS), diffuse reflectance infrared Fourier transform (DRIFT) spectroscopy, fluorescence spectroscopy, direct infusion quadrupole-time of flight mass spectrometry (Q-TOF MS), Escherichia coli N-phenylnaphthylamine (NPN) uptake and bacterial turbidity tests, and Live/DeadRTM and alamarBlueRTM tissue culture assays. Composites with greater than 10% HA by mass were mechanically weak and ruled out as scaffolds. However, 1-5% HA composites were mechanically similar to non-load bearing bone and all resulted in increased osteoblast response at extended time points. The antimicrobial peptide Inverso-CysHHC10 was successfully linked to the 5% HA composite using an interfacial alkene-thiol click reaction. The linked AMP retained its effectiveness against Escherichia coli based on NPN uptake assays and bacterial turbidity tests. Most importantly, the immobilization of the antimicrobial peptide did not affect the increased osteoblast response observed on the unmodified 5% HA. The Inverso-CysHHC10 modified composites present a new class of composite biomaterials that are able to simultaneously address issues with mechanical mismatching, osteoconductivity and implant site infection.
机译：测试了由铝酸钙和羟基磷灰石组成的复合材料作为骨替代支架的适用性。植入的骨支架可能由于多种原因而失效，包括机械和生物学失效。由于周围健康骨骼与支架之间的特性不同，可能会导致机械故障。由于支架和天然骨的界面处的整合问题，或者由于细菌附着和生物膜形成而导致的植入物相关感染，可能导致生物衰竭。研发了钙铝氧化物：羟基磷灰石复合材料，通过材料成分的物理改性和材料界面的化学改性来解决这三种植入失败的模式。;评估了复合材料的相组成，弹性模量，断裂模量，可降解性，成骨细胞附着力，生存力百分比和增殖。使用粉末X射线衍射（PXRD），四点弯曲测试，扫描电子显微镜（SEM），扫描电子显微镜-能量色散X射线光谱（SEM-EDS），漫反射红外傅里叶变换（DRIFT）完成表征光谱，荧光光谱，直接输注四极飞行时间质谱（Q-TOF MS），大肠杆菌N-苯基萘胺（NPN）吸收和细菌浊度测试，以及Live / DeadRTM和alamarBlueRTM组织培养测定。 HA质量含量大于10％的复合材料在机械上较弱，因此被排除为脚手架。但是，1-5％的HA复合材料在机械上与非承重骨骼相似，并且在延长的时间点都导致成骨细胞反应增强。使用界面烯烃-巯基点击反应，将抗菌肽Inverso-CysHHC10成功连接到5％HA复合物。基于NPN摄取分析和细菌浊度测试，连接的AMP保留了针对大肠杆菌的效力。最重要的是，抗菌肽的固定化不会影响在未修饰的5％HA上观察到的成骨细胞应答增加。 Inverso-CysHHC10改性的复合材料提供了一类新型的复合生物材料，能够同时解决机械失配，骨传导性和植入部位感染的问题。

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  • 作者

    Buckholtz, Gavin Andrew.;

  • 作者单位

    Duquesne University.;

  • 授予单位 Duquesne University.;
  • 学科 Biochemistry.;Molecular biology.;Materials science.;Cellular biology.;Biomedical engineering.
  • 学位 Ph.D.
  • 年度 2015
  • 页码 143 p.
  • 总页数 143
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

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