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A Multi-cultural Characterization of Human-environmental Interaction Through Metabolomic and Microbiome Profiling

机译：通过代谢组学和微生物组分析进行人与环境相互作用的多元文化表征

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摘要

Hawaiian culture has long described the value of environmental observation in regards to prolonging human survival. As the human population approaches a staggering 9 billion by the end of the century, human-environmental interaction becomes an important field of study which can provide new insights towards economic, ecological and social management. Advancements in metabolomic and microbiome profiling technology has provided a novel methodology to characterize human-environmental interactions on large scales. The work presented in this dissertation merges indigenous culture and institutionalized science research in order to provide novel methods in molecular exploration.;The first chapter traditionally introduces the author and the importance of Hawaiian culture and institutionalized modern science in today's society. Current metabolome and microbiome profiling methodologies are subsequently introduced in the opening chapter. Chapter 2 describes the spatial mapping of molecular features within the built environment. The microbiome and metabolome of four human volunteers were characterized and mapped across an office space. Annotations made across the metabolome and microbiome provided interesting lifestyle clues to each individual and also help correlate individual to particular sites within the office. Chapter 3 investigates the interactions between three species of Hawaiian coral across a benthic community. Molecular differences across M. capitata, M. flabellatta, and P. lobata were observed. Variance among healthy and disease tissues of M. capitata were also described. Coral molecular features were then mapped onto 3D models, offering new potential for inclusion of 3d molecular profiling into long-term ecological surveys. Chapter 4 explores the relationship between humans and the marine environment by characterizing the metabolome and microbiome of surfers. In additional to identifying that skin is a useful biome to characterize human environmental interaction, the abundance of Psychrobacter on surfer skin in a dose dependent manner to surfing frequency offers new insights towards how our environment shapes our microbiome. Data from the surfer biome project suggests that increase sunscreen use decreases the microbial diversity of human skin. Metabolomic profiling was able to identify key ingredients of sunscreen such as octocrylene which can be further investigated for its effects on microbial diversity. Lastly, chapter 5 describes the future potential for the presented research as well as the importance of science communication.
机译：夏威夷文化长期以来一直描述了环境观察对于延长人类生存的价值。到本世纪末，随着人口接近惊人的90亿，人与环境的相互作用成为重要的研究领域，可以为经济，生态和社会管理提供新的见解。代谢组学和微生物组谱分析技术的进步提供了一种新颖的方法，可以大规模表征人与环境之间的相互作用。本文的工作融合了土著文化和制度化科学研究，以提供分子探索的新方法。第一章传统上介绍了作者以及夏威夷文化和制度化现代科学在当今社会中的重要性。当前的代谢组学和微生物组谱分析方法学随后在开篇中介绍。第2章介绍了构建环境中分子特征的空间映射。对四名人类志愿者的微生物组和代谢组进行了表征，并绘制了整个办公空间的地图。代谢组和微生物组之间的注释为每个人提供了有趣的生活方式线索，还有助于将其与办公室中的特定位置相关联。第三章研究了底栖生物群落中三种夏威夷珊瑚之间的相互作用。观察到了M. capitata，M。flabellatta和P. lobata的分子差异。还描述了M. capitata健康和疾病组织之间的差异。然后将珊瑚分子特征映射到3D模型上，为将3d分子分布图纳入长期生态调查提供了新的潜力。第四章通过描述冲浪者的代谢组和微生物组来探讨人类与海洋环境之间的关系。除了确定皮肤是表征人类环境相互作用的有用生物群系外，冲浪者皮肤上大量的精神细菌对冲浪频率也呈剂量依赖性，这为我们的环境如何塑造微生物组提供了新的见识。冲浪者生物群落项目的数据表明，增加防晒霜的使用会降低人类皮肤的微生物多样性。代谢组学分析能够确定防晒霜的关键成分，例如辛二烯，可以进一步研究其对微生物多样性的影响。最后，第5章描述了本研究的未来潜力以及科学传播的重要性。

著录项

  • 作者

    Kapono, Clifford A.;

  • 作者单位

    University of California, San Diego.;

  • 授予单位 University of California, San Diego.;
  • 学科 Analytical chemistry.
  • 学位 Ph.D.
  • 年度 2018
  • 页码 132 p.
  • 总页数 132
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

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