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首页> 外文学位 >Extending students' practice of metacognitive regulation strategies in the undergraduate chemistry laboratory and investigation of Pb2+ binding to calmodulin with circular dichroism and molecular dynamics modeling.
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Extending students' practice of metacognitive regulation strategies in the undergraduate chemistry laboratory and investigation of Pb2+ binding to calmodulin with circular dichroism and molecular dynamics modeling.

机译：扩展学生在本科化学实验室中的元认知调节策略的实践，并通过圆二色性和分子动力学建模研究Pb2 +与钙调蛋白的结合。

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摘要

The following dissertation was composed of two projects in chemistry education and benchwork/computational biochemistry. The chemistry education research explored students' practice of metacognitive strategies while solving open-ended laboratory problems when engaged in an instructional environment, the Science Writing Heuristic (SWH), that was characterized as supporting metacognitive regulation strategy use. Through in-depth interviews with students, results demonstrated that students in the SWH environment, compared to non-SWH students, used metacognitive strategies to a greater degree and to a greater depth when solving open-ended laboratory problems. As students engaged in higher levels of metacognitive regulation, their elective use of peers became a prominent path for supporting the practice of metacognitive strategies. Students claimed that the structure of the SWH weekly laboratory experiments improved their ability to solve open-ended lab problems. This research not only provided a lens into students' descriptions of their regulation strategy practices in the laboratory, but it also supported that the way that a laboratory environment is arranged can affect these regulation strategy practices and their transfer to new situations.;In the biochemical study on the binding of Pb2+ to calmodulin (CaM), data was acquired via circular dichroism (CD) and molecular dynamics modeling. CD signal data indicated a unique signal from Pb-CaM and a significantly smaller ratio theta208/theta222 for Pb-CaM than Ca-CaM.;An analysis of secondary structure content indicated that alpha-helical structure decreased and random coil structure increased when CaM was saturated with Pb2+ compared to Ca2+ saturated CaM. A molecular dynamics simulation of Pb2+ binding to CaM showed that Pb2+ ions bound to sites outside of the known canonical binding sites including the linker region, and indicated change in secondary structure. These results support the theory of opportunistic binding of Pb 2+ to CaM, and confirm previous research of binding sites in the linker region (Met 76 to Asp 84). The method of random ion (Pb2+ >) placement with a protein (CaM) in a water box provided an accurate prediction of binding sites, correctly predicting four of five binding sites found on both crystal structures of Pb-CaM.
机译：以下论文由化学教育和基准/计算生物化学两个项目组成。化学教育研究探索了学生的元认知策略实践，同时解决了教学环境中的开放式实验室问题，即科学写作启发法（SWH），其特点是支持元认知调节策略的使用。通过对学生的深入访谈，结果表明，与非SWH学生相比，SWH环境中的学生在解决开放式实验室问题时更广泛，更深入地使用了元认知策略。随着学生从事更高水平的元认知调节，他们对同伴的选择性使用成为支持元认知策略实践的重要途径。学生声称，SWH每周实验室实验的结构提高了他们解决开放式实验室问题的能力。这项研究不仅为学生描述了他们在实验室中的调控策略实践提供了视角，而且还支持了实验室环境的安排方式会影响这些调控策略实践及其向新情况的转移。为了研究Pb2 +与钙调蛋白（CaM）的结合，通过圆二色性（CD）和分子动力学建模获得了数据。 CD信号数据表明，来自Pb-CaM的独特信号以及Pb-CaM的theta208 / theta222比Ca-CaM的比率小得多。;对二级结构含量的分析表明，当CaM为与Ca2 +饱和CaM相比，Pb2 +饱和。 Pb2 +与CaM结合的分子动力学模拟表明，Pb2 +离子与已知规范结合位点（包括接头区域）之外的位点结合，并指示二级结构发生了变化。这些结果支持Pb 2+与CaM机会结合的理论，并证实了先前在接头区域（Met 76至Asp 84）的结合位点的研究。在水箱中用蛋白质（CaM）进行随机离子（Pb2 +>）放置的方法可准确预测结合位点，正确预测在Pb-CaM两种晶体结构中发现的五个结合位点中的四个。

著录项

  • 作者

    van Opstal, Mary Twist.;

  • 作者单位

    Loyola University Chicago.;

  • 授予单位 Loyola University Chicago.;
  • 学科 Chemistry Molecular.;Chemistry Biochemistry.;Education Sciences.
  • 学位 Ph.D.
  • 年度 2014
  • 页码 204 p.
  • 总页数 204
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

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