首页> 外文学位 >Morphological and molecular characterization of Libyan olive, Olea europaea L., cultivars (42 local and 16 wild type) in comparison to 41 introduced (world) cultivars.
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Morphological and molecular characterization of Libyan olive, Olea europaea L., cultivars (42 local and 16 wild type) in comparison to 41 introduced (world) cultivars.

机译:利比亚橄榄,欧洲油橄榄(Olea europaea L.)品种(42种本地和16种野生型)与41种(世界)引进品种的形态和分子特征。

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摘要

Olive (Olea europaea L.) consumption and production are important socially and economically in Libya. Olive cultivars that are adapted to local conditions produce olives that have high quality and quantities of oil. Many of the important olive cultivars grown in Libya were evaluated in this research. One goal of this project was to determine the plasticity of morphological traits of olive cultivars that have been grown at diverse locations within Libya. A second goal was to identify a set of traits that are independent of each other and show limited variation (stable traits) regardless of the environmental conditions.;The stable traits were then used in subsequent analyses to correlate genetic and phenotypic characteristics of Libyan olives. Two different groups of olives were compared: the 45 landraces and the 45 cultivars of Olea europaea subsp europaea var. sativa. Morphological data were collected for a total of 39 morphological traits (22 quantitative and 17 qualitative), which were then combined and analyzed to determine phenotypic diversity among different locations.;Differences in many of the morphological traits were observed across the cultivars. These sets of data were used to identify unique and desirable Libyan landraces morphologically. Stable phenotypic traits were used to discriminate between use of fruit (oil or dual-purpose) as well as cultivar origins (local or introduced). This research demonstrates that local Libyan cultivars (landraces) have unique characteristics that differentiate them from imported cultivars.;Ten microsatellite markers were used to differentiate and evaluate the relationships among a total of 91 olive genotypes (39 landraces, 36 introduced cultivars and 16 wild types) collected in Libya. A total of 109 alleles were identified using 10 loci, with the number of alleles per locus ranging from 4 to 20. Three loci (UDO43, DCA16 and GAPU101) had the most alleles with 20, 18 and 16, respectively. The wild types and introduced cultivars had greater numbers of alleles than the local cultivars. Six cases of duplicated genotypes, two cases of synonymy, and thirteen homonyms that were genetically distinct were observed in the Libyan collection.;UPGMA clustering classified the accessions into two main distinct groups. The first group consisted of landraces and the second group included introduced cultivars and wild type accessions. Admixture analysis also distinguished between landraces and wild genotypes. In general, molecular data enables one to separate the Libyan olive accessions based on their orgin but not on their fruit use.
机译:橄榄(Olea europaea L.)的消费和生产在利比亚具有重要的社会和经济意义。适应当地条件的橄榄品种生产的橄榄品质高且油量高。这项研究对利比亚种植的许多重要橄榄品种进行了评估。该项目的目标之一是确定在利比亚境内不同地点种植的橄榄品种形态特征的可塑性。第二个目标是确定一组相互独立的特征,无论环境条件如何,其变异(稳定的特征)均有限。然后将稳定的特征用于随后的分析中,以利比亚橄榄的遗传和表型特征相关联。比较了两组不同的橄榄:45种地方品种和45种油橄榄(Olea europaea subsp europaea var)。苜蓿。收集了39种形态性状的形态学数据(22个定量性和17个定性性状),然后进行合并和分析以确定不同位置之间的表型多样性。;在不同品种之间观察到许多形态性状的差异。这些数据集用于从形态上识别独特且理想的利比亚地方品种。稳定的表型性状用于区分使用水果(油或双重用途)和品种起源(本地或引进)。这项研究表明,利比亚本地品种(地方品种)具有与进口品种区别的独特特征。;使用十个微卫星标记来区分和评估总共91个橄榄基因型之间的关系(39个地方品种,36个引入品种和16种野生类型) )收集在利比亚。使用10个基因座共鉴定了109个等位基因,每个基因座的等位基因数量为4至20。三个基因座(UDO43,DCA16和GAPU101)的等位基因最多,分别为20、18和16。野生型和引进品种比当地品种具有更多的等位基因。在利比亚收藏中观察到6例重复的基因型,2例同义和13例在遗传上不同的同音异义词。UPGMA聚类将种质分为两个主要的不同组。第一组包括地方品种,第二组包括引进的品种和野生型种质。混合分析还区分了地方品种和野生基因型。通常,分子数据使人们能够根据其来源而不是根据其水果用途来分离利比亚橄榄种。

著录项

  • 作者

    Abdul Sadeg, Salem.;

  • 作者单位

    Colorado State University.;

  • 授予单位 Colorado State University.;
  • 学科 Agriculture Horticulture.;Biology Genetics.;Biology Anatomy.
  • 学位 Ph.D.
  • 年度 2014
  • 页码 131 p.
  • 总页数 131
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

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