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首页> 外文学位 >Newt lens regeneration: Role of oct-4 in newt regenerating tissue and proteome analysis of regeneration competent vs. regeneration incompetent cells.
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Newt lens regeneration: Role of oct-4 in newt regenerating tissue and proteome analysis of regeneration competent vs. regeneration incompetent cells.

机译：t晶状体再生：oct-4在new再生组织中的作用以及再生感受态与非再生感受态细胞的蛋白质组分析。

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摘要

Newts possess remarkable ability for regenerating various body organs and parts. Lens regeneration in newts is achieved by transdifferentiation of dorsal iris to lens cells. This ability of transdifferentiation is exhibited only from the dorsal iris and not by the ventral iris. In order to study the process of transdifferentiation during lens regeneration and the characteristics that allow lens regeneration only from the dorsal iris, we studied the role of reprogramming factor; oct-4 during newt lens regeneration and analyzed the proteome of dorsal iris cells vs. ventral iris cells respectively. During transdifferentiation, the dorsal iris has to change its cellular identity in order to achieve identity of lens cells. Such mechanisms are also observed during generation of iPS cells and previous studies have shown that these two processes share some similarities. It was observed that one of the crucial reprogramming factor oct-4 was absent in the regenerating tissues of newt. Thus, we hypothesize that absence of oct-4 during newt lens regeneration restricts the IPE cells to only a particular fate (lens cells). We over expressed oct-4 in the newt IPE cells and studied its effect on transdifferentiation potential of these cells. The results showed oct-4 inhibits the process of transdifferentiation in newt dorsal IPE cells by interfering with sox-2 and pax-6, factors important for synthesis of crystallin during lens formation. Thus, oct-4 absence during newt lens regeneration allows the dorsal iris to change its cellular identity to regenerate the lost lens. Further, we also examined the proteome of cultured regeneration competent dorsal IPE cells and regeneration incompetent ventral iris cells with a goal to identify any molecular marker specific to either dorsal or ventral IPE cells. The proteome was studied using newt's de novo assembled transcriptome as a reference library. The results did not reveal any specific markers for either of the cell population. However, on comparison of in vitro proteome to in vivo proteome of 0, 4 and 8 day regenerating iris identified some factors related to gene regulation and retinoic acid synthesis with higher expression in the dorsal IPE cells compared to the ventral IPE cells.
机译：ts具有惊人的再生各种身体器官和部位的能力。 ts中的晶状体再生通过背虹膜向晶状体细胞的转分化来实现。仅从背侧虹膜表现出这种转分化能力，而腹侧虹膜则没有表现出这种能力。为了研究晶状体再生过程中的转分化过程，以及仅允许从背虹膜再生晶状体的特性，我们研究了重编程因子的作用。 oct-4在new晶状体再生过程中，并分别分析了背虹膜细胞与腹虹膜细胞的蛋白质组。在转分化过程中，背虹膜必须改变其细胞身份以实现晶状体细胞的身份。在iPS细胞生成过程中也观察到了这种机制，先前的研究表明这两个过程具有一些相似之处。观察到在t的再生组织中不存在关键的重编程因子oct-4之一。因此，我们假设在lens晶状体再生期间不存在oct-4会将IPE细胞限制为特定的命运（晶状体细胞）。我们在the IPE细胞中过表达oct-4，并研究了其对这些细胞的转分化潜能的影响。结果表明，oct-4通过干扰sox-2和pax-6（在晶状体形成过程中对结晶蛋白合成很重要的因素）来抑制new背IPE细胞的转分化过程。因此，在new晶状体再生期间不存在oct-4可使背虹膜改变其细胞身份以再生丢失的晶状体。此外，我们还检查了培养的再生感受态背侧IPE细胞和再生能力不佳的腹侧虹膜细胞的蛋白质组，目的是鉴定任何特定于背侧或腹侧IPE细胞的分子标记。该蛋白质组是使用newt的de novo组装转录组作为参考库进行研究的。结果没有揭示任何一种细胞群的特异性标记。然而，在体外蛋白质组与体内蛋白质组的0、4和8天再生虹膜的比较中，确定了一些与基因调节和视黄酸合成有关的因素，与腹侧IPE细胞相比，背IPE细胞中的表达更高。

著录项

  • 作者

    Bhavsar, Rital B.;

  • 作者单位

    University of Dayton.;

  • 授予单位 University of Dayton.;
  • 学科 Cellular biology.
  • 学位 Dr.Ph.
  • 年度 2014
  • 页码 102 p.
  • 总页数 102
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 人类学;
  • 关键词

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