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首页> 外文学位 >Discourse on synthesis and characterization of bimetallic complexes of oxygen/nitrogen donor ligands as structural and functional models of the active site of xylose/glucose isomerase.
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Discourse on synthesis and characterization of bimetallic complexes of oxygen/nitrogen donor ligands as structural and functional models of the active site of xylose/glucose isomerase.

机译：讨论氧/氮供体配体的双金属配合物的合成和表征，作为木糖/葡萄糖异构酶活性位点的结构和功能模型。

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摘要

This research presents and discusses the synthesis and characterization of structural and functional model complexes of Xylose/Glucose Isomerases (XGI). The XGI enzyme is a metal-ion activated bacterial enzyme that catalyzes the stereospecific interconversion of D-xylose to D-xylulose and D-glucose to D-fructose. However, very little is known about the mode of substrate binding or mechanism of action. This project focuses on an original systematic design, synthesis, and characterization of models which mimic the structural and functional nature of XGI. Several structural bimetallic model ligands of XGI were synthesized using organic reactions. These ligands were then reacted with metal ions of interest to produce bimetallic model complexes. Binding of the carbohydrate substrates in a specific stereochemistry to the bimetallic modeled complex was demonstrated using UV-vis spectroscopic technique at different reaction conditions. The specific mode of binding to the complexes has been elucidated using NMR spectroscopy. Additionally, molecular calculations were performed to reaffirm suspected modes of substrate binding to the model complexes. The data indicated that both D-glucose and D-xylose to interact through 1,2-binding to the bimetallic complexes.
机译：这项研究提出并讨论了木糖/葡萄糖异构酶（XGI）的结构和功能模型复合物的合成和表征。 XGI酶是一种金属离子活化的细菌酶，催化D-木糖向D-木酮糖和D-葡萄糖向D-果糖的立体定向互变。但是，关于底物结合的方式或作用机理知之甚少。该项目致力于模拟XGI的结构和功能特性的模型的原始系统设计，合成和表征。利用有机反应合成了XGI的几种结构双金属模型配体。然后使这些配体与感兴趣的金属离子反应以产生双金属模型络合物。使用紫外可见光谱技术在不同的反应条件下证明了碳水化合物底物在特定的立体化学中与双金属模型的复合物的结合。使用NMR光谱已经阐明了与复合物结合的特定模式。另外，进行分子计算以确认底物与模型复合物结合的可疑模式。数据表明D-葡萄糖和D-木糖都通过1，2-结合至双金属配合物相互作用。

著录项

  • 作者

    Curtiss, Ashley Brian Sockwell.;

  • 作者单位

    The University of Texas at San Antonio.;

  • 授予单位 The University of Texas at San Antonio.;
  • 学科 Chemistry Inorganic.
  • 学位 Ph.D.
  • 年度 2009
  • 页码 114 p.
  • 总页数 114
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

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