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首页> 外文学位 >ERK 1/2 and p38 MAPK pathways are both invovled in the expression of interleukin-6, -8, and cyclooxygenase-2 in thrombocytes stimulated with lipopolysaccharide.
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ERK 1/2 and p38 MAPK pathways are both invovled in the expression of interleukin-6, -8, and cyclooxygenase-2 in thrombocytes stimulated with lipopolysaccharide.

机译：ERK 1/2和p38 MAPK通路均与脂多糖刺激的血小板中白细胞介素6，-8和环氧合酶2的表达有关。

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摘要

This study was conducted to determine if the p38 and ERK1/2 MAPK pathways are involved in transcription of IL-6, IL-8, and COX-2 in the chicken thrombocyte response to ligation of Toll-like receptor 4 (TLR4) by Salmonella minnesota lipopolysaccharide (LPS). Thrombocytes were isolated and subsequently treated with either p38 MAPK or ERK inhibitor, and then with LPS during in vitro cell culture. Transcription of IL-6, IL-8, and COX-2 mRNA was determined using real-time PCR. The experiments were repeated using pM, nM and muM concentrations of both inhibitors to test cell sensitivity. Stimulation with LPS induced expression of IL-6, IL-8 and COX-2 mRNA. This expression was reduced by treatment with 25 muM ERK inhibitor as well as by treatment with 70 muM p38 MAPK inhibitor. However, treatment with 25 pM and nM ERK inhibitor, and with 70 pM and nM p38 MAPK inhibitor, yielded no decrease in mRNA expression in relation to treatment with LPS alone. Inhibition with both the ERK and p38 MAPK inhibitors was in accordance with previously established concentrations for mammalian cells. It is now apparent that the p38 and ERK1/2 MAPK pathways are both linked to gene expression of a cytokine, a chemokine and an enzyme involved in the thrombocyte innate response to TLR4 ligation by bacterial LPS.
机译：进行这项研究来确定p38和ERK1 / 2 MAPK通路是否参与沙门氏菌对Toll样受体4（TLR4）的连接反应中鸡血小板中IL-6，IL-8和COX-2的转录明尼苏达州脂多糖（LPS）。分离血小板，随后在体外细胞培养过程中用p38 MAPK或ERK抑制剂处理，然后用LPS处理。使用实时PCR测定IL-6，IL-8和COX-2 mRNA的转录。使用两种抑制剂的pM，nM和muM浓度重复实验以测试细胞敏感性。用LPS刺激诱导IL-6，IL-8和COX-2mRNA的表达。通过用25μMERK抑制剂治疗以及通过用70μMp38 MAPK抑制剂治疗，该表达降低。但是，与单独使用LPS治疗相比，用25 pM和nM ERK抑制剂以及70 pM和nM p38 MAPK抑制剂治疗不会产生mRNA表达下降。 ERK和p38 MAPK抑制剂的抑制作用均与先前确定的哺乳动物细胞浓度一致。现在很明显，p38和ERK1 / 2 MAPK途径都与细胞因子，趋化因子和酶的基因表达有关，这些因子参与了细菌LPS对TLR4结扎的血小板固有反应。

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  • 作者

    Hitchcock, Candace M.;

  • 作者单位

    Clemson University.;

  • 授予单位 Clemson University.;
  • 学科 Biology Microbiology.;Health Sciences Immunology.
  • 学位 M.S.
  • 年度 2009
  • 页码 58 p.
  • 总页数 58
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

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