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首页> 外文学位 >Characterization of biosynthetic and catabolic pathways of Bacillus subtilis strain 168.
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Characterization of biosynthetic and catabolic pathways of Bacillus subtilis strain 168.

机译：枯草芽孢杆菌菌株168的生物合成和分解代谢途径的表征。

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摘要

Bacillus subtilis has long been a model bacterium for understanding biological mechanisms, such as fatty acid catabolism and polyketide biosynthesis. Our interest in the latter was centered on the polyketide synthase (PKS) mechanism responsible for beta-branching polyketides. The unique structural moiety is attributed to a HMG-CoA synthase homolog, such as the pksG gene in B. subtilis..;The first goal was a metagenomic survey of local soils, using the conserved pksG homolog sequence as a genetic marker. After optimizing techniques for the extraction and purification of environmental DNA, the beta-branching polyketide population was not detected in any local soil samples. While working with a pksG homolog, an apparent sequence anomaly prompted us to verify the taxonomic classification of B. subtilis research strains ATCC 39374 and 39320. Comparison of DNA sequences ( pksG homologs, hypervariable regions of 16S rRNA and rDNA) and species-specific genes showed the two ATCC strains are more closely related to B. amyloliquefaciens. .;A group of genes named the mmg operon, able to catalyze fatty acid catabolism, are active only during B. subtilis sporulation. Our hypothesis was that, by creating a conditional genetic knockout mutation, the bacterium would be capable of in-vitro growth in propionate media. Attempts to subclone portions of mmg DNA were unsuccessful. However, the neighboring YqiQ protein was successfully isolated and purified.
机译：枯草芽孢杆菌长期以来一直是了解生物机制的模型细菌，例如脂肪酸分解代谢和聚酮化合物的生物合成。我们对后者的兴趣集中在负责β分支聚酮化合物的聚酮化合物合酶（PKS）机制上。独特的结构部分归因于HMG-CoA合酶同源物，例如枯草芽孢杆菌中的pksG基因。第一个目标是使用保守的pksG同源序列作为遗传标记，对当地土壤进行宏基因组学调查。优化提取和纯化环境DNA的技术后，在任何本地土壤样品中均未检测到β-支化聚酮化合物。当使用pksG同源物时，一个明显的序列异常促使我们验证枯草芽孢杆菌研究菌株ATCC 39374和39320的分类学分类。DNA序列（pksG同源物，16S rRNA和rDNA的高变区）和物种特异性基因的比较结果表明，两种ATCC菌株与解淀粉芽孢杆菌的亲缘关系更近。一组名为mmg操纵子的基因，能够催化脂肪酸分解代谢，仅在枯草芽孢杆菌形成孢子期间才有活性。我们的假设是，通过产生条件性基因敲除突变，该细菌将能够在丙酸介质中体外生长。尝试亚克隆mmg DNA的部分失败。但是，相邻的YqiQ蛋白已成功分离和纯化。

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  • 作者

    Quattlebaum, Amy L.;

  • 作者单位

    The University of North Carolina at Greensboro.;

  • 授予单位 The University of North Carolina at Greensboro.;
  • 学科 Chemistry Biochemistry.
  • 学位 M.S.
  • 年度 2009
  • 页码 95 p.
  • 总页数 95
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

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