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Verifying transcript up-regulation for three proteins under nitogen depletion in Chlamydomonas reinhardtii.

机译：验证莱茵衣藻中氮素耗竭下三种蛋白质的转录本上调。

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摘要

Using the algae such as Chlamydomonas reinhardtii production of biofuel is growing. Under the nitrogen depletion, Chlamydomonas reinhardtii show a large amount of lipid accumulation. The lipids that accumulate in the algae are in the form of triacylglycerols (TAGs). Collaborators at the University of Nebraska-Lincoln found that some proteins showed up-regulation during lipid accumulation. A 14-3-3 protein, a putative ammonium transporter, and a glycerol 3-phosphate dehydrogenase were three of them. The aim of this study was to verify the change of transcript levels of these three proteins under the nitrogen depletion in Chlamydomonas reinhardtii strains cc124 and cc125. In this project, COX4 served as the negative control and DGTT1 as the positive control when qRT-PCR was performed. For DGTT1, the 14-3-3 protein, and the ammonium transporter, cc125 has a higher up-regulation than cc124, but for the glycerol-3-phosphate dehydrogenase the opposite was the case. In general, all of the assays for both strains did show clear up-regulation. These results indicate that the transcripts of DGTT1, a 14-3-3 protein, an ammonium transporter, and a glycerol-3-phosphate dehydrogenase may play a role in the TAG synthesis required for lipid accumulation in Chlamydomonas reinhardtii under nitrogen deprivation.
机译：使用诸如莱茵衣藻（Chlamydomonas reinhardtii）等藻类的生物燃料生产正在增长。在氮耗竭下，莱茵衣藻显示了大量的脂质积累。藻类中积累的脂质为三酰基甘油（TAG）的形式。内布拉斯加州大学林肯分校的合作者发现，某些蛋白质在脂质蓄积期间显示出上调的作用。 14-3-3蛋白，推定的铵转运蛋白和3-磷酸甘油脱氢酶是其中的三个。这项研究的目的是验证莱茵衣藻cc124和cc125在氮耗竭下这三种蛋白质的转录水平变化。在该项目中，当进行qRT-PCR时，COX4用作阴性对照，DGTT1用作阳性对照。对于DGTT1、14-3-3蛋白和铵转运蛋白，cc125具有比cc124更高的上调，但对于甘油3-磷酸脱氢酶则相反。通常，两种菌株的所有测定法均显示出明显的上调。这些结果表明DGTT1，14-3-3蛋白，铵转运蛋白和3-磷酸甘油脱氢酶的转录本可能在缺乏氮的情况下在莱茵衣藻中脂质积累所需的TAG合成中起作用。

著录项

  • 作者

    Wang, Li.;

  • 作者单位

    University of Nebraska at Kearney.;

  • 授予单位 University of Nebraska at Kearney.;
  • 学科 Microbiology.
  • 学位 M.S.
  • 年度 2014
  • 页码 66 p.
  • 总页数 66
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 教育;
  • 关键词

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