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首页> 外文学位 >Characterization of Strain PTV-2 USA/IA65463/2014 and Strain PTV-11 USA/IA09592/2013 of Teschovirus A: Experimental Inoculation, Distribution of Nucleic Acids and Development of Teschovirus Encephalomyelitis =Caracterización de las cepas PTV-2 USA/IA65463
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Characterization of Strain PTV-2 USA/IA65463/2014 and Strain PTV-11 USA/IA09592/2013 of Teschovirus A: Experimental Inoculation, Distribution of Nucleic Acids and Development of Teschovirus Encephalomyelitis =Caracterización de las cepas PTV-2 USA/IA65463

机译：细小病毒A的PTV-2菌株USA / IA65463 / 2014和细小菌株PTV-11的USA / IA09592 / 2013的表征：细小病毒的实验接种，核酸的分布和发展脊髓脊髓炎=拉斯凯帕氏菌（Caracterizaciónde las cepas）PTV-2 USA / IA65463

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摘要

Teschovirus A (previously Porcine teschovirus; PTV) is the etiologic agent of Teschovirus encephalomyelitis (TE). Historically, PTV-1 has been recognized as one of the most pathogenic serotypes, although other serotypes have been described to cause TE. Hence, most information about the pathogenicity, epidemiology and control of disease relies on data from studies involving PTV-1.;In recent years, two cases submitted to the Iowa State University Veterinary Diagnostic Laboratory with a history of polioencephalitis and/or myelitis were identified and genomic sequencing characterized these isolates as (sero)types PTV-2 and PTV-11.;Although multiple serotypes have been identified and genetically characterized, the neuropathogenicity of some of these serotypes has not been fully elucidated.;To assess these isolates, we developed an experimental model to determine the neuropathogenicity of the PTV-2 and PTV-11 isolates and observed that both isolates caused histological lesions and clinical disease consistent with TE in cesarean-derived colostrum-deprived pigs. Furthermore, PTV RNA was detected in different tissues, serum and feces in all inoculated animals by RT-qPCR.;The experimental approach used in this research permitted to develop a successful platform to induce clinical disease. This is the first description of a neuropathogenic PTV-11 strain in the U.S. and the first experimental inoculation using a PTV-2 autochthonous U.S. strain after the initial description of PTV-2 strain O3b made by Long at al. in 1966. This is also the first assessment of the viral shedding, viremia and distribution by real time RT-qPCR of nucleic acids of PTV-2 and PTV-11 in experimentally infected pigs with Teschovirus encephalomyelitis.
机译：Teschovirus A（以前是猪teschovirus； PTV）是Teschovirus脑脊髓炎（TE）的病原体。从历史上看，PTV-1被认为是致病性最强的血清型之一，尽管其他血清型也被描述为可引起TE。因此，有关致病性，流行病学和疾病控制的大多数信息都依赖于涉及PTV-1的研究数据。近年来，发现了两例提交至爱荷华州立大学兽医诊断实验室的病例，其中有脊髓灰质炎和/或脊髓炎病史基因分离和基因组测序将这些分离株鉴定为（血清）型PTV-2和PTV-11。虽然已鉴定出多种血清型并进行了遗传学表征，但尚未完全阐明其中一些血清型的神经致病性。为评估这些分离株，我们他开发了一个实验模型来确定PTV-2和PTV-11分离株的神经致病性，并观察到这两种分离株在剖宫产初乳剥夺猪中均引起与TE一致的组织学损害和临床疾病。此外，通过RT-qPCR在所有接种动物的不同组织，血清和粪便中检测到PTV RNA。该研究中使用的实验方法为开发成功的诱导临床疾病的平台提供了条件。这是Long等人对PTV-2菌株O3b的最初描述之后，这是美国首次对神经致病性PTV-11菌株进行的描述，也是首次使用PTV-2本地美国菌株进行的实验接种。也是在1966年。这是通过实时RT-qPCR对实验感染猪瘟病毒性脑脊髓炎的猪中PTV-2和PTV-11核酸进行病毒脱落，病毒血症和分布的首次评估。

著录项

  • 作者

    Matias Ferreyra, Franco S.;

  • 作者单位

    Iowa State University.;

  • 授予单位 Iowa State University.;
  • 学科 Veterinary science.;Virology.
  • 学位 M.S.
  • 年度 2017
  • 页码 100 p.
  • 总页数 100
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

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