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首页> 外文学位 >The effects of breeding density, year, and laboratory vs. field environments on plasma and yolk steroids in green anole lizards ( Anolis carolinensis).
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The effects of breeding density, year, and laboratory vs. field environments on plasma and yolk steroids in green anole lizards ( Anolis carolinensis).

机译：繁殖密度，年份以及实验室和田间环境对绿色无尾蜥蜴（Anolis carolinensis）血浆和蛋黄类固醇的影响。

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摘要

Scope and method of study. My objectives were to: (1) determine how breeding density and year affect plasma and yolk T and CORT concentrations in a laboratory environment; and (2) document female plasma and yolk T and CORT concentrations in a field environment. Breeding females were housed in cages with either one male (low density) or one male and three additional females (high density) to evaluate the effects of breeding density on reproductive effort and plasma and yolk testosterone (T) and corticosterone (CORT) concentrations. All laboratory animals were maintained under standard breeding conditions for ten weeks over the course of two breeding seasons. Nest boxes were checked daily for eggs which were frozen for subsequent yolk steroid analyses. At the end of the study, blood samples were collected from all laboratory animals. Blood samples and eggs were collected from wild-caught females. All blood and yolk samples were analyzed using radioimmunoassay.;Findings and conclusions. I found that breeding density did not affect male initial body condition, but did affect male final body condition and change in body condition. A significant year effect was seen in male initial, final, and change in body condition. Breeding density did not affect female body condition, but year effects were seen in female initial and change in body condition. Egg mass was affected by year, but not breeding density. Surprisingly, females in low density cages produced more eggs than females in high density cages and year also affected egg production. Female and male plasma CORT and T concentrations were unaffected by breeding density or year, except males in high breeding densities tended to have higher plasma T concentrations and females in 2008 had higher plasma CORT concentrations than females in 2007. Yolk T and CORT concentrations were affected by year and yolk CORT concentrations tended to be higher in eggs from high breeding densities, rather than low breeding densities. In comparison with field data, eggs from females in the laboratory were heavier, but had lower levels of yolk T and CORT. Wild-caught females tended to have greater mass and SVL than laboratory females, but laboratory females had higher concentrations of plasma CORT.
机译：研究范围和方法。我的目标是：（1）确定实验室环境中的繁殖密度和年份如何影响血浆和蛋黄T和CORT浓度； （2）记录田间环境中的女性血浆，卵黄T和CORT浓度。将雌性雌性关在笼子里，雄性（低密度）或雄性和三头雌性（高密度）一起关在笼子里，以评估繁殖密度对生殖力以及血浆和蛋黄睾丸激素（T）和皮质酮（CORT）浓度的影响。在两个繁殖季节的过程中，将所有实验动物在标准繁殖条件下饲养十周。每天检查巢箱中的鸡蛋，然后将其冷冻以进行蛋黄类固醇分析。在研究结束时，从所有实验动物中采集血液样本。从野外捕获的雌性中采集血样和卵。使用放射免疫分析法分析了所有血液和蛋黄样品。发现和结论。我发现繁殖密度不会影响男性的初始身体状况，但会影响男性的最终身体状况和身体状况的变化。在男性的最初，最后和身体状况变化中可以看到明显的年份效应。繁殖密度没有影响女性的身体状况，但是在女性初期和身体状况的变化中可以看到年效应。蛋重受年份影响，但不受繁殖密度影响。出乎意料的是，低密度网箱中的雌性卵比高密度网箱中的雌性卵更多，一年也影响了卵的生产。雌性和雄性血浆的CORT和T浓度不受繁殖密度或年份的影响，除了高繁殖密度的雄性的血浆T浓度较高，而2008年的雌性的血浆CORT浓度高于2007年的雌性。卵黄T和CORT浓度受到影响逐年增加，高繁殖密度而不是低繁殖密度的卵中蛋黄CORT浓度往往更高。与现场数据相比，实验室中的雌性卵较重，但卵黄T和CORT含量较低。野外捕获的雌性往往比实验室雌性具有更大的质量和SVL，但实验室雌性的血浆CORT浓度较高。

著录项

  • 作者

    Adams, Amber Lynn.;

  • 作者单位

    Oklahoma State University.;

  • 授予单位 Oklahoma State University.;
  • 学科 Animal Physiology.
  • 学位 M.S.
  • 年度 2009
  • 页码 57 p.
  • 总页数 57
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

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