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首页> 外文学位 >Effects of zilpaterol and melengestrol acetate on bovine skeletal muscle growth and development.
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Effects of zilpaterol and melengestrol acetate on bovine skeletal muscle growth and development.

机译：齐帕特罗和醋酸美仑孕酮对牛骨骼肌生长发育的影响。

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摘要

Zilpaterol (ZIL) is a beta-adrenergic receptor (beta-AR) agonist that has been recently approved for use in feedlot cattle to improve production efficiencies and animal performance. One of the mechanisms through which this occurs is increased skeletal muscle growth. Therefore, two experiments were conducted to determine the effects of ZIL both in vivo and in vitro. In the first experiment, ZIL addition to bovine satellite cells resulted in a tendency to increase IGF-I mRNA and increased myosin heavy chain IIA (MHC) mRNA with 0.001 muM and decreased MHC mRNA with 0.01 and 10 muM. There were no effects of ZIL on protein synthesis or degradation. In myoblast cultures, there was a decrease in all three beta-AR mRNA, and this was also reported in western blot analysis with a reduction in beta2-AR expression due to ZIL treatment. In myotubes, there was an increase in beta2-AR protein expression. In the second and third experiment, ZIL improved performance and carcass characteristics of feedlot steers and heifers. Additionally, ZIL decreased MHC IIA mRNA in semimembranosus muscle tissue collected from both steers and heifers. An additional part of the third study was conducted to determine the effects of melengestrol acetate (MGA) on bovine satellite cell and semimembranosus muscle gene expression. There were no effects of MGA on the expression of genes analyzed from semimembranosus muscle tissue collected. However, the addition of MGA to cultured bovine satellite cells resulted in increased beta1 and beta2-AR mRNA. These experiments aid in our understanding of the mechanism of action of MGA in heifers, as well as the effects of ZIL on both steers and heifers. Furthermore, they increase our knowledge and understanding of the mechanism of action of ZIL, as well as other beta-agonists used to promote growth and efficiency in feedlot animals.
机译：齐帕特罗（ZIL）是一种β-肾上腺素能受体（β-AR）激动剂，最近被批准用于肥育场的牛，以提高生产效率和动物性能。发生这种情况的机制之一是增加骨骼肌的生长。因此，进行了两个实验以确定ZIL在体内和体外的作用。在第一个实验中，向牛卫星细胞中添加ZIL导致IGF-I mRNA升高，肌球蛋白重链IIA（MHC）mRNA升高（0.001μM）和MHC mRNA降低（0.01和10μM）的趋势。 ZIL对蛋白质合成或降解没有影响。在成肌细胞培养物中，所有三个β-ARmRNA均降低，并且在Western印迹分析中也有报道，由于ZIL处理，β2-AR表达降低。在肌管中，β2-AR蛋白表达增加。在第二个和第三个实验中，ZIL改善了育肥场ste牛和小母牛的性能和car体特性。此外，ZIL减少了从ste牛和小母牛身上收集的半膜肌组织中的MHC IIA mRNA。进行了第三项研究的另一部分，以确定乙酸美仑孕酮（MGA）对牛卫星细胞和半膜肌基因表达的影响。 MGA对从收集的半膜肌组织中分析的基因表达没有影响。但是，向培养的牛卫星细胞中添加MGA会导致beta1和beta2-AR mRNA增加。这些实验有助于我们了解MGA在小母牛中的作用机理，以及ZIL对ste牛和小母牛的影响。此外，它们增加了我们对ZIL作用机理以及用于促进饲养场动物生长和效率的其他β激动剂的了解和了解。

著录项

  • 作者

    Sissom, Erin Kathryn.;

  • 作者单位

    Kansas State University.;

  • 授予单位 Kansas State University.;
  • 学科 Agriculture Animal Culture and Nutrition.
  • 学位 Ph.D.
  • 年度 2009
  • 页码 155 p.
  • 总页数 155
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 饲料;
  • 关键词

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