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首页> 外文学位 >Isolation and identification of the fecal pollution indicators Enterococcus spp. from seagrass Thalassia testudinum at La Parguera Puerto Rico and the use of enterococci for microbial source tracking.
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Isolation and identification of the fecal pollution indicators Enterococcus spp. from seagrass Thalassia testudinum at La Parguera Puerto Rico and the use of enterococci for microbial source tracking.

机译：粪便污染指标肠球菌的分离与鉴定。来自波多黎各La P​​arguera的海草Thalassia testudinum，以及使用肠球菌进行微生物来源跟踪。

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摘要

The occurrence and persistence of the fecal contamination indicators Enterococcus spp. were studied in La Parguera, southwestern Puerto Rico, as an initial step in microbial source tracking (MST). The scientific literature suggests that different enterococcal species are associated to specific homeothermic hosts. Results from this study indicate birds as the main sources of fecal pollution in the area and suggests a E. gallinarum - cattle egret association. Precipitation may be a major factor in enterococcal occurrence. There are also reports that indicate that enterococci might survive for extended periods of time in sediments and in the phylosphere of vegetation. Potential non-point sources of enterococci may cause an overestimation of the enterococci that suggest fecal pollution as well as an obstacle for MST. In consequence, the association and survival of enterococci in the seagrass Thalassia testudinum, was evaluated. It was found that T. testudinum is able to host enterococci. However, overtime persistence of enterococci in this ecosystem was not evident. During the development of this study, high numbers of false positive enterococci were found while using standard methods for the isolation of enterococci from the environment. A protocol using Enterococcosel Broth followed by two confirmation tests was examined as a potential approach to be used in monitoring coastal waters of Puerto Rico. The method presented is economically viable, but still needs improvement. A re-evaluation of the current methods for fecal contamination assessment in our coastline is suggested.
机译：粪便污染指标肠球菌的出现和持续。在波多黎各西南部的La Parguera进行了研究，这是微生物来源跟踪（MST）的第一步。科学文献表明，不同的肠球菌物种与特定的等温寄主有关。这项研究的结果表明，鸟类是该地区粪便污染的主要来源，并暗示了鸡大肠杆菌-牛背鹭协会。沉淀可能是肠球菌发生的主要因素。也有报告表明肠球菌可能在沉积物和植被的系统层中存活更长的时间。肠球菌的潜在面源可能导致对肠球菌的高估，这表明粪便污染以及MST的障碍。结果，评估了海草地中海藻睾丸中肠球菌的结合和存活。已经发现，睾丸丙酸杆菌能够宿主肠球菌。然而，肠球菌在该生态系统中的持续时间不明显。在进行这项研究的过程中，发现大量假阳性肠球菌，同时使用标准方法从环境中分离出肠球菌。一项使用肠球菌肉汤并随后进行两次确认试验的方案被检查为监测波多黎各沿海水域的一种潜在方法。提出的方法在经济上可行，但仍需要改进。建议对我们海岸线上目前的粪便污染评估方法进行重新评估。

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  • 作者

    Rivera-Torres, Yaritza.;

  • 作者单位

    University of Puerto Rico, Mayaguez (Puerto Rico).;

  • 授予单位 University of Puerto Rico, Mayaguez (Puerto Rico).;
  • 学科 Biology Microbiology.;Biology Oceanography.;Environmental Sciences.
  • 学位 M.S.
  • 年度 2009
  • 页码 94 p.
  • 总页数 94
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 微生物学;环境科学基础理论;海洋生物;
  • 关键词

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