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首页> 外文学位 >Development and evaluation of a high-speed, continuous-flow, automated plasmapheresis procedure for the collection of equine plasma according to current good manufacturing practices.
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Development and evaluation of a high-speed, continuous-flow, automated plasmapheresis procedure for the collection of equine plasma according to current good manufacturing practices.

机译：根据当前的良好生产规范，开发和评估高速，连续流，自动血浆分离程序，以收集马血浆。

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摘要

The purpose of this investigation was to develop and evaluate a high-speed, continuous-flow, automated plasmapheresis procedure for the collection of equine plasma according to current good manufacturing practices. Once a safe and reliable method was achieved, the plasmapheresis procedure was used to perform serial donations. The effect of serial plasmapheresis on total plasma protein and total IgG concentrations was evaluated throughout the investigation.;Adaptations were made to human-model automated plasmapheresis instruments and sterile collection sets, which allowed for dual-instrument, continuous-flow operation. Equine donors were connected to the modified collection sets via jugular vein catheters. The instruments extracted whole blood, infused an anticoagulant, fractionated the anticoagulated whole blood, harvested the plasma and returned the concentrated blood cells to the donors.;The procedures lasted between 3 to 6 hours in length and yielded approximately 20L of plasma per 900kg horse. During the investigation, 3,240 plasmapheresis donations were performed, 143 different horses were plasmapheresed and more than 50,000L of plasma were collected. The majority of donors tolerated the procedure and remained calm during plasmapheresis. Adverse events associated with the procedure were minor, occurred with an extremely low frequency or were eliminated after making adjustments to the technique.;The described plasmapheresis procedure was used to harvest 22ml of plasma per kg donor body weight at 14-day intervals. Donors experienced a statistically significant (p < 0.05) decrease in both total plasma protein and total IgG concentrations during the investigation. However, the decreases in concentration were not physiologically significant. Neither total protein nor total IgG concentrations were below the reference interval at the time of donation. Additionally, the concentrations did not decrease further with serial donations, but stabilized at a reduced concentration or returned to baseline. Despite the decreases in IgG concentration, the donors did not demonstrate an increase in disease incidence. These results indicate that the described serial plasmapheresis protocol allowed time for donor horses to replace lost plasma proteins and IgG.;A safe and reliable method for automated equine plasmapheresis was achieved. The collection of 22ml of plasma per kg donor body weight at 14-day intervals did not cause sustained total protein or IgG depletion in the donors.
机译：这项研究的目的是根据当前的良好生产规范开发和评估高速，连续流，自动血浆分离术，以收集马血浆。一旦实现了安全可靠的方法，就使用血浆置换程序进行系列捐赠。在整个研究过程中，评估了连续血浆置换对总血浆蛋白和总IgG浓度的影响。对人体模型自动血浆置换仪器和无菌采集套件进行了调整，从而可以进行双仪器，连续流操作。马供体通过颈静脉导管连接到改良的收集装置。仪器提取全血，注入抗凝剂，分馏抗凝全血，收集血浆并将浓缩的血细胞返还给供体。该过程持续3至6小时，每900kg马产生约20L血浆。在调查过程中，进行了3​​,240次血浆置换捐赠，对143匹不同的马进行了血浆置换，并收集了超过50,000升血浆。大多数捐献者耐受该过程，并在血浆置换过程中保持镇定。与该程序相关的不良事件很小，发生频率极低，或者在对技术进行调整后就消除了。上述血浆置换程序用于每14天间隔每公斤供体体重收获22ml血浆。在研究过程中，捐赠者的血浆总蛋白和总IgG浓度均有统计学显着性下降（p <0.05）。但是，浓度的降低在生理上并不明显。捐赠时总蛋白和总IgG浓度均未低于参考区间。此外，浓度不会随着连续捐赠而进一步降低，而是稳定在降低的浓度或恢复到基线水平。尽管IgG浓度降低，但供体并未显示出疾病发生率增加。这些结果表明，所描述的串行血浆置换术方案允许供体马有时间替换丢失的血浆蛋白和IgG。；实现了一种安全可靠的自动马血浆置换术方法。每14天一次每公斤供体体重收集22ml血浆，不会导致供体中总蛋白或IgG持续消耗。

著录项

  • 作者

    Ziska, Sara Marie.;

  • 作者单位

    Auburn University.;

  • 授予单位 Auburn University.;
  • 学科 Biology Veterinary Science.;Health Sciences Immunology.
  • 学位 Ph.D.
  • 年度 2009
  • 页码 175 p.
  • 总页数 175
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

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