• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文学位 >Development of droplet-based microfluidic devices for microdroplet trapping and pairing.
【24h】

Development of droplet-based microfluidic devices for microdroplet trapping and pairing.

机译：基于微滴的微滴捕获和配对的微滴装置的开发。

获取原文
获取原文并翻译 | 示例
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

The droplet based microfluidic technology has become indispensable in many chemical, biomedical research and high-throughput assay applications. The ability to controllably merge droplets within flow systems is of high importance when performing complex chemical or biological analysis. However, in order to perform controlled fusion reaction one needs to perform controlled droplet trapping and pairing. Recent microfluidic systems are capable of pairing the droplets by using unstabilized flow pattern. Controlled droplet pairing and fusion, especially for same-sized droplet pairing, is still a challenge, mostly because of the difficulty to manipulate droplets. It is also seen that it requires to control the droplet generation along with the flow rate control simultaneously which is also difficult to realize. In our research, a serial flowing microfluidic system and an obstruction based microfluidic system are presented for checking the droplet flow pattern along the system using hydrodynamic resistance phenomenon. In addition to this, we also checked the device working for droplet generation along with sequential trapping and pairing of aqueous micro-droplets of different liquids. It is more robust as compared to the prior research done in this area.;These systems are competent of accomplishing multiple functions including droplet generation, transportation, trapping and merging on a single integrated device. These devices consist of three different functional regions: flow focusing droplet generator; a single droplet trap region and pairing region. Our designs were based on the principle of exploiting hydrodynamic resistance of the columnar structure in the microfluidic channel. The device designs include two inlets for oil and water. Similar structure was embedded at the outlet for the generation of second droplet of different liquid. In a typical scenario, droplets would be generated at the T-junction and would travel through the microfluidic channel to enter the single droplet trapping area. During the reverse flow, the trapped droplets in the first phase would be released and would enter the pairing chamber. These droplets would be held until another droplet of different liquid to combine with it. Second droplet would travel in the reverse flow direction and would be trapped in the pairing chamber along with the first droplet to combine with it. Deionized water and gel were used as the aqueous phase and mineral oil as the oil phase. 2% (w/w) Span-80 was used as surfactant. These devices were also simulated using PSpice and COMSOL Multiphysics to verify the droplet trapping and pairing sequences before fabrication.;Finally, we designed and tested the double droplet trapping system in a serial flowing microfluidic device along with the obstruction based microfluidic device. The efficiency for single droplet trapping in forward flow was about 99%, single droplet trapping in reverse flow direction was about 90-95% for both serial and obstruction based microfluidic device. For droplet pairing, the serial microfluidic device had an efficiency of 40-45% where as the obstruction based microfluidic had 60-65% efficiency. These devices were very simple and could very efficiently trap two different liquid droplets in a chamber without merging and with the help of an external electric field they could be selectively merged.
机译：基于液滴的微流技术已成为许多化学，生物医学研究和高通量分析应用中不可缺少的技术。当执行复杂的化学或生物学分析时，可控地合并液滴在流动系统中的能力非常重要。然而，为了进行受控的融合反应，需要进行受控的液滴捕获和配对。最近的微流体系统能够通过使用不稳定的流型来配对液滴。受控的液滴配对和融合，尤其是对于相同大小的液滴配对，仍然是一个挑战，主要是因为难以操纵液滴。还可以看出，它需要与流量控制同时控制液滴的产生，这也是很难实现的。在我们的研究中，提出了串行流动微流体系统和基于阻塞的微流体系统，以利用流体动力阻力现象检查沿系统的液滴流动模式。除此之外，我们还检查了用于产生液滴的设备，以及依次捕获和配对不同液体的水性微小液滴的装置。与该领域的现有研究相比，它更健壮。这些系统能够在单个集成设备上完成多种功能，包括液滴生成，传输，捕获和合并。这些设备由三个不同的功能区域组成：流动聚焦液滴发生器；单个液滴捕获区和配对区。我们的设计基于利用微流体通道中柱状结构的流体动力阻力的原理。该设备设计包括两个油和水入口。在出口处嵌入类似的结构，以产生不同液体的第二滴。在典型情况下，液滴将在T形结处生成，并穿过微流体通道进入单个液滴捕获区域。在反向流动期间，在第一阶段捕获的液滴将被释放并进入配对室。这些液滴将一直保持到另一滴不同液体与之合并。第二液滴将沿相反的流动方向行进，并且将与第一液滴一起被捕获在配对室中并与之结合。去离子水和凝胶用作水相，矿物油用作油相。 2％（w / w）Span-80用作表面活性剂。还使用PSpice和COMSOL Multiphysics对这些设备进行了仿真，以在制造之前验证液滴的捕获和配对顺序。最后，我们在串行流动的微流体设备以及基于阻塞的微流体设备中设计并测试了双液滴捕获系统。对于基于串行和基于阻塞的微流体装置，在向前流动中捕获单个液滴的效率约为99％，在反向流动方向捕获单个液滴的效率约为90-95％。对于液滴配对，串行微流控设备的效率为40-45％，而基于阻塞的微流控设备的效率为60-65％。这些设备非常简单，可以非常有效地将两个不同的液滴捕获在一个腔室内而不会合并，并且借助外部电场可以选择性地合并它们。

著录项

  • 作者

    Gopalan, Preethi.;

  • 作者单位

    State University of New York at Buffalo.;

  • 授予单位 State University of New York at Buffalo.;
  • 学科 Engineering Electronics and Electrical.;Engineering Materials Science.
  • 学位 M.S.
  • 年度 2010
  • 页码 116 p.
  • 总页数 116
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号