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首页> 外文学位 >Photoluminescence spectroscopy of bioconjugated quantum dots and their application for early cancer detection.
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Photoluminescence spectroscopy of bioconjugated quantum dots and their application for early cancer detection.

机译：生物共轭量子点的光致发光光谱及其在早期癌症检测中的应用。

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摘要

Most of the bio-applications of semiconductor quantum dots (QDs) show and utilize their superior optical properties over organic fluorophores. An estimated 3--35% of all cancer deaths could be avoided through early detection, therefore, there is a critical need to develop sensitive probes.;The objectives of this work are: (1) Research the phenomena of "blue" photoluminescence (PL) spectral shift on the dried bioconjugated QDs and develop the relevant mechanism; (2) Develop a methodology that will allow successful confirmation of the bioconjugation reaction between biomolecules and QDs; (3) Propose a modification of an existent method or approach to employ the "blue" spectral shift of bioconjugated QDs for early cancer detection.;Results indicated that the "blue" spectral shift, observed for dried on the silicon substrates bioconjugated QDs, is increased with the time of storage and reaches 30-40nm in 14 days. It is accelerated at elevated temperatures and slowed down at lower temperatures. Larger size QDs generate spectral shifts of larger magnitudes, and the spectral shift is positively correlated with the biomolecule's size/weight. This phenomenon is explained by elastic and compression stress due to nonhomogenious drying of the QD droplet and the reaction with the solid surface.;Agarose gel electrophoresis technique, optimized with organic dye fluorescamine, is suitable for bioconjugation verification. The optimal running parameters were found to be 2% agarose gel, 1.5V working voltage, 0.5X TBE as a running buffer, and about 120 mins running time.;The spectral shift was implemented for improving the sensitivity of Prostate Specific Antigen (PSA) Enzyme-Linked ImmunoSorbent Assay (ELISA). It was found that QD ELISA could be as much, as 100 times more sensitive than the regular commercial ELISA, based on the enzymatic detection.;The results of this work show that QDs may be very useful for early detection of several types of cancers, including prostate cancer in men and breast/ovarian/uterine cancers in women.
机译：半导体量子点（QD）的大多数生物应用都显示并利用了其优于有机荧光团的光学特性。通过早期发现，可以避免所有癌症死亡的大约3--35％，因此，迫切需要开发灵敏的探针。该工作的目标是：（1）研究“蓝色”光致发光现象（ PL）在干燥的生物共轭QD上发生光谱偏移并建立相关机制; （2）开发一种方法，以成功确认生物分子与量子点之间的生物结合反应； （3）提出对现有方法或方法的修改，以采用生物共轭QD的“蓝”光谱位移进行早期癌症检测。结果表明，在硅基板上生物共轭QD干燥后观察到的“蓝”光谱位移为随着储存时间的增加，在14天内达到30-40nm。在高温下会加速，在低温下会减速。较大尺寸的量子点会产生较大幅度的光谱偏移，并且光谱偏移与生物分子的尺寸/重量成正相关。 QD液滴的不均匀干燥以及与固体表面的反应会导致弹性和压缩应力，从而解释了这种现象。琼脂糖凝胶电泳技术经过有机染料荧光胺的优化，适用于生物缀合验证。发现最佳的运行参数为2％琼脂糖凝胶，1.5V工作电压，0.5X TBE作为运行缓冲液，以及约120分钟的运行时间。;实施了光谱偏移以提高前列腺特异性抗原（PSA）的敏感性酶联免疫吸附测定（ELISA）。基于酶促检测，发现QD ELISA的灵敏度可能是普通商业ELISA的100倍。；这项工作的结果表明，QD可能对早期检测多种类型的癌症非常有用，包括男性的前列腺癌和女性的乳腺癌/卵巢癌/子宫癌。

著录项

  • 作者

    Chornokur, Ganna.;

  • 作者单位

    University of South Florida.;

  • 授予单位 University of South Florida.;
  • 学科 Engineering Biomedical.
  • 学位 Ph.D.
  • 年度 2009
  • 页码 154 p.
  • 总页数 154
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

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