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首页> 外文学位 >Design and construction of phospholipid membrane bilayer biomimetic devices for fundamental understanding of physical and chemical membrane processes .
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Design and construction of phospholipid membrane bilayer biomimetic devices for fundamental understanding of physical and chemical membrane processes .

机译：设计和构造磷脂膜双层仿生装置的基础和物理和化学膜过程的了解。

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摘要

Over the past century, the scientific community has spent a great deal of effort trying to understand the cellular membrane bilayer on an intimate level. Langmuir deposition and vesicle fusion has provided a means to deposit membrane bilayers atop solid inorganic supports. These simple membranes remain fluid in nature due to a 10-30 A water cushion that rests between the membrane and the solid substrate. Although these mimetic templates are ideal for simple lipid membrane, placement of membrane proteins into these systems results in loss of membrane fluidity, and the proteins often become denatured. It has been suggested that portions of the embedded protein extend beyond the vertical length of the membrane and water cushion, and negatively interact with the inorganic support. Due to these substrate-protein interactions, polymeric cushions and tethers have been used to adjust the vertical distance between the membrane and solid support. Poly(ethylene glycol) (PEG) amphiphiles were one of the first polymers used to remedy the substrate-protein interactions because of its hydrophilic and bioinert qualities. It was found that membranes tethered with PEG amphiphiles actually have up to three different motilities within a single system. There has been much speculation as to why these systems contain different motilities, but no real studies have been conducted to assess the issue. One focus of this dissertation was to study PEG-amphiphile/lipid interactions at the air-water interface. During the process of characterizing these binary mixtures, an interesting meta-stable domain changing phenomena was found to occur at low surface pressures within a narrow concentration regime in these mixtures. These serendipitous results will be described.;Due to the different mobile fractions within a single PEG cushioned/tethered membrane system, other polymers have been explored. In this work the development of pH responsive, hydrophilic, and cross-linked poly (acrylic acid) (PAA) based cushioned membrane bilayers are also depicted. The cellular membranes own cushion inspired the design of these cushions; the cytoskeleton, which is a netted network that is able to change shape, based on environmental changes. In addition Gramicidin A transmembrane protein was embedded into PAA cushioned membrane bilayers.
机译：在过去的一个世纪中，科学界付出了巨大的努力，试图从亲密的角度理解细胞膜双层。 Langmuir沉积和囊泡融合提供了一种将膜双层沉积在固体无机载体上的方法。这些简单的膜由于位于膜和固体基质之间的10-30 A水垫而在本质上保持流体流动。尽管这些模拟模板是简单脂质膜的理想选择，但将膜蛋白放置在这些系统中会导致膜流动性丧失，并且蛋白经常会变性。已经提出，包埋的蛋白质的部分延伸超过膜和水垫的垂直长度，并且与无机载体负作用。由于这些底物-蛋白质相互作用，已使用聚合物垫和系绳来调节膜和固体支持物之间的垂直距离。聚乙二醇（PEG）两亲物由于具有亲水性和生物惰性特性，是最早用于修复底物与蛋白质相互作用的聚合物之一。发现与PEG两亲物束缚的膜在单个系统中实际上具有多达三个不同的功能。关于这些系统为何包含不同的功能的猜测很多，但是还没有进行真正的研究来评估这个问题。本文的重点之一是研究空气-水界面处的PEG-两亲/脂质相互作用。在表征这些二元混合物的过程中，发现在这些混合物的窄浓度范围内，低表面压力下会发生有趣的亚稳态畴变化现象。将描述这些偶然的结果。由于在单个PEG缓冲/系留膜系统中存在不同的移动部分，因此已经探索了其他聚合物。在这项工作中，还描述了基于pH响应，亲水性和交联聚（丙烯酸）（PAA）的缓冲膜双层的发展。蜂窝膜本身的坐垫启发了这些坐垫的设计。细胞骨架，这是一个网状网络，能够根据环境变化来改变形状。另外，葛兰素A跨膜蛋白被嵌入PAA缓冲膜双层中。

著录项

  • 作者

    El-khouri, Rita Jaqueline.;

  • 作者单位

    University of California, Davis.;

  • 授予单位 University of California, Davis.;
  • 学科 Chemistry Polymer.;Biophysics General.
  • 学位 Ph.D.
  • 年度 2009
  • 页码 200 p.
  • 总页数 200
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

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