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首页> 外文学位 >Three daughters of NG2: Lineage tracing of NG2+ progenitors using a transgenic mouse model.
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Three daughters of NG2: Lineage tracing of NG2+ progenitors using a transgenic mouse model.

机译：NG2的三个女儿：使用转基因小鼠模型对NG2 +祖细胞进行谱系追踪。

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摘要

Studying the development of the mammalian central nervous system has been a great challenge because of its complexity and the difficulty in finding appropriate model systems. Through years of research, many protein makers have been identified and associated with certain neural lineages at specific developmental stage. However, the specificity and reliability of many protein markers used have been challenged in recent years. The transmembrane protein, neural-glial 2 (NG2), has been considered the cellular marker expressed in oligodendrocyte precursor cells (OLPs), but recent reports have suggested that postnatal NG2+ OLPs have multiple cell fates. To conclusively determine the differentiation potentials of NG2+ OLPs, I applied Cre/LoxP lineage tracing strategy starting with generating the NG2-Cre transgenic mouse line with using the BAC transgenic method. The NG2-Cre mice were then crossed with the reporter mouse line, Rosa26LacZ. By tracing the expression of transgene, I have confirmed that NG2+ progenitor cells are capable to generate all three neural cell lineages instead of one as previously believed. Furthermore, lineage tracing of NG2 progenitors also indicated that the cell lineage determination is partially controlled by microenvironmental factors, since NG2 progenitors' differentiation potential is region specific. Although, microenvironmental stimulation is critical for cell differentiation, but it did not seem to be the only factor that influence cell specification. By comparing two double transgenic mouse lines, (NG2-Cre/Rosa26LacZ and Gfap-Cre/Rosa26LacZ), I have found that both NG2 and GFAP progenitor cells can give rise to granule neurons in the dentate gyms, but the terminal location of two neuronal populations were distinctively generated.
机译：由于其复杂性和寻找合适的模型系统的困难，研究哺乳动物中枢神经系统的发展一直是一个巨大的挑战。通过多年的研究，已经确定了许多蛋白质制造商，并在特定的发育阶段与某些神经谱系相关联。然而，近年来使用的许多蛋白质标记的特异性和可靠性受到挑战。跨膜蛋白神经胶质2（NG2）被认为是在少突胶质前体细胞（OLP）中表达的细胞标志物，但最近的报道表明出生后的NG2 + OLP具有多种细胞命运。为了最终确定NG2 + OLP的分化潜力，我应用了Cre / LoxP谱系追踪策略，首先使用BAC转基因方法生成了NG2-Cre转基因小鼠品系。然后将NG2-Cre小鼠与报告基因小鼠Rosa26LacZ杂交。通过追踪转基因的表达，我已经证实NG2 +祖细胞能够生成所有三个神经细胞谱系，而不是以前认为的一个。此外，NG2祖细胞的谱系追踪还表明，由于NG2祖细胞的分化潜力是区域特异性的，因此细胞谱系的确定部分受微环境因素控制。尽管微环境刺激对于细胞分化至关重要，但它似乎并不是影响细胞规格的唯一因素。通过比较两个双转基因小鼠系（NG2-Cre / Rosa26LacZ和Gfap-Cre / Rosa26LacZ），我发现NG2和GFAP祖细胞均可在齿状体育馆中产生颗粒神经元，但两个神经元的末端位置人口是独特产生的。

著录项

  • 作者

    Tsoa, Rosemarie Wen-Ting.;

  • 作者单位

    University of California, Los Angeles.;

  • 授予单位 University of California, Los Angeles.;
  • 学科 Biology Molecular.;Biology Neuroscience.
  • 学位 Ph.D.
  • 年度 2009
  • 页码 176 p.
  • 总页数 176
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

  • 入库时间 2022-08-17 11:38:30

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