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Transcriptional regulation of Legionella pneumophila intracellular multiplication.

机译：嗜肺军团菌细胞内增殖的转录调控。

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摘要

Legionella pneumophila has a unique intracellular lifestyle characterized by the formation of a specialized compartment that supports bacterial replication. Legionella species replicate in unicellular protozoa in the environment and alveolar macrophages in susceptible human hosts. Replication within alveolar macrophages can result in the severe and often fatal pneumonia Legionnaires' disease. Intracellular survival and growth require that L. pneumophila manipulate host endocytic trafficking by an unusual process indicative of complex regulation. Genes associated with L. pneumophila intracellular multiplication encode a secretion system, translocated effector proteins, and regulators of gene expression. Here, I investigate L. pneumophila intracellular growth through the analysis of transcriptional regulators. The alternative sigma factor, sigma S is used as a tool to investigate Legionella transcriptional networks and identify regulators required for intracellular multiplication. I determined that sigmaS controls multiple pathways associated with the intracellular growth of Legionella. I also found that the transcription factor ArgR is required for intracellular multiplication and controls transcription in response to L-arginine. Evidence is presented that L-arginine availability affects gene expression during intracellular growth. Thus, this thesis contributes to our understanding of the transcriptional regulation of L. pneumophila intracellular multiplication.
机译：嗜肺军团菌具有独特的细胞内生活方式，其特征是形成了支持细菌复制的专门区室。军团菌物种在环境中的单细胞原生动物中复制，而易感人宿主中的肺泡巨噬细胞则复制。在肺泡巨噬细胞内复制可导致严重的，甚至致命的肺炎军团病。细胞内生存和生长需要嗜肺乳杆菌通过指示复杂调控的异常过程来操纵宿主内吞运输。与嗜肺乳杆菌细胞内增殖相关的基因编码分泌系统，易位的效应蛋白和基因表达的调节剂。在这里，我通过对转录调节子的分析来研究嗜肺乳杆菌的细胞内生长。另一种sigma因子sigma S用作研究军团菌转录网络并确定细胞内增殖所需的调节子的工具。我确定sigmaS控制与军团菌细胞内生长相关的多种途径。我还发现，转录因子ArgR是细胞内增殖所必需的，并且可响应L-精氨酸来控制转录。证据表明，L-精氨酸的可用性影响细胞内生长过程中的基因表达。因此，本论文有助于我们理解嗜肺乳杆菌的细胞内增殖的转录调控。

著录项

  • 作者

    Hovel-Miner, Galadriel A.;

  • 作者单位

    Columbia University.;

  • 授予单位 Columbia University.;
  • 学科 Biology Microbiology.
  • 学位 Ph.D.
  • 年度 2009
  • 页码 158 p.
  • 总页数 158
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

  • 入库时间 2022-08-17 11:38:31

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