• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文学位 >Evaluation of novel live and inactivated Salmonella - Vectored vaccines for immunization of chicks in-ovo and at hatch.
【24h】

Evaluation of novel live and inactivated Salmonella - Vectored vaccines for immunization of chicks in-ovo and at hatch.

机译：新型活和灭活沙门氏菌的评估-用于卵内和孵化雏鸡免疫的载体疫苗。

获取原文
获取原文并翻译 | 示例
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

As we previously reported, an attenuated DeltaaroA/Delta htrA Salmonella enteritidis strain (DeltaSE ) expressing two M2e epitopes and an abbreviated CD154 sequence on the bacterial cell surface provided high, persistent, and neutralizing antibody titers against several influenza serotypes when administered post-hatch. The present studies evaluate in ovo administration of DeltaSE on hatchability and antibody production. In addition, clearance of DeltaSE from liver, spleen, and cecal tonsils was evaluated. At day 18E chicken embryos were vaccinated with 0.25 mL of 3.25 x 103, 2.5 x 105, and 2.7 x 105 cfu DeltaSE/embryo (air cell) in experiments 1, 2, and 3, respectively. Control groups were injected with 0.25 mL sterile saline. Chicken hatchability was not affected in these experiments and no clinical signs of disease were detected in any experiment. Liver, spleen, and cecal tonsils were aseptically removed from chicks and cultured for DeltaSE recovery. Serum M2e- and DeltaSE-specific IgG antibody response was measured at specific sampling periods for each experiment. Serological levels for IgG anti-M2e antibodies increased during the first week of age. However, IgG anti-DeltaSE antibodies were not detected at any of the sampling times. Clearance of DeltaSE from liver and spleen and cecal tonsils was greatly diminished by the end of each experiment. Although a brief immune response was observed at 1 week post-vaccination, no antibody against M2e was observed at later times. These data suggest that in ovo administration of this vectored M2e-based vaccine is not a potentially effective means of prophylaxis in commercial poultry.;The objective of the subsequent studies were to evaluate the effect of an inactivated a Salmonella enteritidis recombinant vaccine (DeltaSE) expressing surface type A influenza M2e epitopes on the immune response of broiler chickens. Treatment groups consisted of heat- (1 hour at 65°C) or formaldehyde- (0.05%) inactivation (10 7 cfu/mL) alone or in combination with an adjuvant (aluminum hydroxide; 1 or 2.6 mg/dose) and were evaluated by ELISA for antibody against DeltaSE or M2e. In Experiments 1 and 2, day-of-hatch broilers were divided into groups (n=50), neck tagged, placed in a common pen, and injected with 0.2 mL saline (control), or heat- or formaldehyde-inactivated recombinant bacteria with or without adjuvant at day-of-hatch, and with or without booster vaccination at day 21 post-hatch. Blood samples were collected and body weights were measured at approximately 10 day intervals in both experiments. No effect on performance was observed, and anti-M2e responses were not significant between groups at any age, although significantly p 0.05 increased antibodies against DeltaSE was observed Although previous studies have indicated that live DeltaSE inoculation in day-of-hatch chicks results in marked increase in M2e antibody levels, the present study indicates that parenteral administration of this vectored vaccine is unlikely to provide protective immune response against avian influenza when inactivated or adjuvated by these methods.
机译：如我们先前所报道的，在孵化后施用时，在细菌细胞表面表达两个M2e表位和CD154缩写序列的减毒DeltaaroA / Delta htrA肠炎沙门氏菌菌株（DeltaSE）提供了针对几种流感血清型的高，持久和中和抗体效价。本研究评估卵在DeltaSE的孵化率和抗体产生上的管理。此外，还评估了DeltaSE从肝脏，脾脏和盲肠扁桃体中的清除率。在第18E天，分别在实验1、2和3中分别给0.25 mL的3.25 x 103、2.5 x 105和2.7 x 105 cfu DeltaSE /胚胎（空气细胞）接种鸡胚。对照组注射0.25mL无菌盐水。在这些实验中不影响鸡的孵化率，并且在任何实验中均未检测到疾病的临床症状。从小鸡中无菌取出肝，脾和盲肠扁桃体，并培养以回收DeltaSE。在每个实验的特定采样时间测量血清M2e和DeltaSE特异性IgG抗体反应。 IgG抗M2e抗体的血清学水平在年龄的第一周内增加。但是，在任何采样时间均未检测到IgG抗DeltaSE抗体。每次实验结束时，肝脏，脾脏和盲肠扁桃体中DeltaSE的清除率大大降低。尽管在接种后1周观察到短暂的免疫反应，但在以后的时间里未观察到针对M2e的抗体。这些数据表明，在卵内施用这种载体化的基于M2e的疫苗并不是预防商业家禽的潜在有效手段。;后续研究的目的是评估灭活表达沙门氏菌肠炎沙门氏菌重组疫苗（DeltaSE）的效果表面A型流感M2e表位对肉鸡的免疫反应。治疗组由热（在65°C下1小时）或甲醛（0.05％）（10 7 cfu / mL）灭活（单独或与佐剂（氢氧化铝； 1或2.6 mg /剂量）组合）组成，并进行了评估ELISA检测抗DeltaSE或M2e的抗体。在实验1和2中，将孵化日的肉鸡分成组（n = 50），进行颈部标记，放在普通钢笔中，并注射0.2 mL盐水（对照），或者用热或甲醛灭活的重组细菌孵化后有或没有佐剂，孵化后第21天有或没有加强免疫。在两个实验中，以大约10天的间隔收集血样并测量体重。没有观察到对性能的影响，并且在任何年龄段的组之间抗M2e反应均不显着，尽管观察到的针对DeltaSE的抗体明显增加了p <0.05。尽管先前的研究表明，孵化日雏鸡的活DeltaSE接种可导致如果M2e抗体水平显着增加，则本研究表明，通过这些方法灭活或灭活后，这种载体疫苗的肠胃外给药不太可能提供针对禽流感的保护性免疫应答。

著录项

  • 作者

    Gaona-Ramirez, Guillermo Antonio.;

  • 作者单位

    University of Arkansas.;

  • 授予单位 University of Arkansas.;
  • 学科 Biology Microbiology.;Agriculture Animal Culture and Nutrition.;Agriculture Animal Pathology.
  • 学位 Ph.D.
  • 年度 2009
  • 页码 86 p.
  • 总页数 86
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 微生物学;动物医学（兽医学）;饲料;
  • 关键词

  • 入库时间 2022-08-17 11:38:27

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号