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首页> 外文学位 >The regulation and function of SoxB1 genes and proteins during neural induction and development in Xenopus laevis.
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The regulation and function of SoxB1 genes and proteins during neural induction and development in Xenopus laevis.

机译：非洲爪蟾神经诱导和发育过程中SoxB1基因和蛋白的调节和功能。

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摘要

In Xenopus laevis, ectodermal cells are induced to form neural tissue when BMP signaling is inhibited. Little is known about the steps following inhibition of BMP that allows for the expression of early neural genes such as sox2, sox3 and geminin. This research investigates the regulation and function of the SoxB1 genes and proteins which are activated by BMP inhibition during neural induction in Xenopus. We use gain and loss of function assays to determine that BMP signaling inhibits the expression of the soxB1 gene, sox3 via its downstream targets, XVent1 and XVent2. Additionally, we demonstrate that FGF signaling is required for the induction of sox2, but is only required for the maintenance of sox3 and geminin. Our data indicates that although the same signals and factors are involved in the expression and repression of early neural genes, there is no common neurogenic code regulating neural induction in Xenopus laevis. Moreover, since the soxB1 genes are expressed at the onset of neural induction and are required for normal neural development, we analyze their role in neural induction and formation. We demonstrate that overexpression of Sox2 and Sox3 directly activates expression of the neural progenitor marker, geminin, thereby expanding the neural tube and delaying neuronal differentiation. This progenitor expansion occurs at the expense of epidermis and neural crest formation indicating that the SoxB1 proteins are involved in the fate choice between epidermis and neural tissue and they act to promote neurogenesis at the expense of non-neural ectodermal derivatives.
机译：在非洲爪蟾中，当BMP信号被抑制时，外胚层细胞被诱导形成神经组织。抑制BMP后允许早期神经基因如sox2，sox3和geminin表达的步骤知之甚少。这项研究调查了非洲爪蟾在神经诱导过程中被BMP抑制激活的SoxB1基因和蛋白质的调节和功能。我们使用功能丧失和功能测定来确定BMP信号通过其下游靶标XVent1和XVent2抑制soxB1基因sox3的表达。此外，我们证明FGF信号传导是诱导sox2所必需的，但仅是维持sox3和geminin所必需的。我们的数据表明，尽管相同的信号和因素参与了早期神经基因的表达和抑制，但在非洲爪蟾中没有共同的调节神经诱导的神经源性代码。此外，由于soxB1基因在神经感应开始时就表达，是正常神经发育所必需的，因此我们分析了它们在神经感应和形成中的作用。我们证明Sox2和Sox3的过度表达直接激活神经祖细胞标记物geminin的表达，从而扩大神经管并延迟神经元分化。该祖细胞的扩增以表皮和神经c的形成为代价，这表明SoxB1蛋白参与表皮和神经组织之间的命运选择，并且它们以非神经外胚层衍生物为代价来促进神经发生。

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  • 作者

    Rogers, Crystal D.;

  • 作者单位

    Georgetown University.;

  • 授予单位 Georgetown University.;
  • 学科 Biology Molecular.
  • 学位 Ph.D.
  • 年度 2009
  • 页码 163 p.
  • 总页数 163
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 分子遗传学;
  • 关键词

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