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首页> 外文学位 >Strategies for the analysis of biologically active compounds by capillary electrophoresis and microchip-capillary electrophoresis.
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Strategies for the analysis of biologically active compounds by capillary electrophoresis and microchip-capillary electrophoresis.

机译：通过毛细管电泳和微芯片-毛细管电泳分析生物活性化合物的策略。

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摘要

Sensitive, selective, and cost-effective analysis of biomolecules (phenols, amino acids, etc.) is important in clinical, environmental, and industrial fields. Capillary electrophoresis (CE) is one of the most used analytical techniques offering high performance, reagent economy, speed, automation capabilities, and miniaturization. At the microchip scale, CE also provides custom design, reduced consumption of reagents and sample, low waste generation, increased analysis speed, and portability. In this proposal the analysis by microchip-CE was divided in two main steps: the separation step and the detection step. The main goal of this dissertation was to investigate different strategies to improve the analytical performance of both of these steps. Based in the stated goal, the specific aims of the current dissertation were to investigate: (1) the electrophoretic/electrochemical effects of different surface coatings. (2) the interaction of selected enzymes with nanomaterials. Regarding the dynamic modification of the capillary surface, a very simple method for the coating of fused silica capillaries with a natural clay was developed. Some remarkable advantages of this coating procedure include stability, constant mu EOF at alkaline pH values, low cost of the reagents employed, and the possibility of using it in microchip devices. On the other side, the electrophoretic effects of surfactants with different chain lengths were also studied as a dynamic modification for the separation of six phenolic compounds. As a result of the adsorption of surfactants to the PDMS surface, reductions in the migration times and improvements in peak skew (S) were observed for all the surfactants when compared to bare PDMS. Surfactants offer a convenient and versatile approach to decrease analyte-wall interactions, improve peak shape, and decrease analysis time. Regarding the electrochemical detection, the addition of anionic surfactants can also produce improvements in the electrochemical detection of some phenolic compounds by enhancing interactions between the analyte and the electrode surface.
机译：对生物分子（酚，氨基酸等）进行灵敏，选择性和经济高效的分析在临床，环境和工业领域中非常重要。毛细管电泳（CE）是最常用的分析技术之一，可提供高性能，试剂经济性，速度，自动化功能和小型化。在微芯片规模上，CE还可以提供定制设计，减少试剂和样品的消耗，减少废物产生，提高分析速度和便携性。在该提议中，通过微芯片-CE进行的分析分为两个主要步骤：分离步骤和检测步骤。本文的主要目的是研究提高这两个步骤的分析性能的不同策略。基于所述目标，本论文的具体目的是研究：（1）不同表面涂层的电泳/电化学作用。 （2）选定的酶与纳米材料的相互作用。关于毛细管表面的动态改性，开发了一种非常简单的用天然粘土涂覆熔融石英毛细管的方法。这种包衣方法的一些显着优点包括稳定性，在碱性pH值下恒定的mu EOF，所用试剂的低成本以及在微芯片设备中使用它的可能性。另一方面，还研究了不同链长的表面活性剂的电泳效果，将其作为动态改性以分离六种酚类化合物。表面活性剂吸附到PDMS表面的结果是，与裸露的PDMS相比，所有表面活性剂的迁移时间均减少，峰偏斜率（S）有所提高。表面活性剂提供了一种方便且通用的方法来减少分析物与壁的相互作用，改善峰形并减少分析时间。关于电化学检测，阴离子表面活性剂的添加还可以通过增强分析物与电极表面之间的相互作用来改善某些酚类化合物的电化学检测。

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  • 作者

    Mora, Maria Fernanda.;

  • 作者单位

    The University of Texas at San Antonio.;

  • 授予单位 The University of Texas at San Antonio.;
  • 学科 Chemistry Analytical.
  • 学位 Ph.D.
  • 年度 2009
  • 页码 158 p.
  • 总页数 158
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

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