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首页> 外文学位 >Functional interaction between PIPKIalpha and the non-canonical poly(A) polymerase star-PAP for the efficient 3' end formation of select mRNAs.
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Functional interaction between PIPKIalpha and the non-canonical poly(A) polymerase star-PAP for the efficient 3' end formation of select mRNAs.

机译：PIPKIalpha和非经典的poly（A）聚合酶star-PAP之间的功能相互作用，可实现选定mRNA的有效3'端形成。

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摘要

Phosphoinositides are a family of lipid signalling molecules that act to regulate a number of cellular functions. By identifying proteins which interact with phosphoinositide generating enzymes, great strides have been made revealing mechanisms for phosphoinositide signalling events. This study was undertaken to determine mechanisms for the nuclear phosphoinositide signalling pathways that require the phosphoinositide kinase PIPKIalpha and its product PI4,5P2.;Star-PAP was identified as a direct interacting protein with PIPKIalpha and the enzyme activity of Star-PAP is regulated by PI4,5P2. Star-PAP embodies a unique architecture and its domain arrangement is distinct compared to any known nucleotidyl transferase enzyme. Star-PAP resides in a large protein complex that contains a number of factors required for the 3' end processing of mRNAs, though is devoid of detectable canonical PAP. Unlike the canonical PAP complex, Star-PAP contains PIPKIalpha which is enzymatically active, as well as the phosphoinositide sensitive protein kinase Casein Kinase Ialpha, defining a unique phosphoinositide nuclear complex that is acts to regulate select gene expression.;The collected data to be presented define molecular mechanisms for the regulation of 3' end mRNA processing regulated by nuclear phosphoinositides and demonstrates that nuclear phosphoinositide based signalling pathways function to regulate mRNA levels through controlling 3' end formation of mRNA processing.
机译：磷酸肌醇是脂质信号分子的家族，其起着调节许多细胞功能的作用。通过鉴定与磷酸肌醇生成酶相互作用的蛋白质，已经取得了长足的进步，揭示了磷酸肌醇信号传导事件的机制。进行了这项研究以确定需要磷酸肌醇激酶PIPKIalpha及其产物PI4,5P2的核磷酸肌醇信号传导途径的机制; Star-PAP被鉴定为与PIPKIalpha的直接相互作用蛋白，而Star-PAP的酶活性受PI4,5P2。 Star-PAP体现了独特的结构，与任何已知的核苷酸转移酶相比，其结构域排列都不同。 Star-PAP驻留在一个大型蛋白质复合物中，该复合物中含有许多mRNA的3'末端加工所需的因子，尽管缺少可检测的规范PAP。与典型的PAP复合物不同，Star-PAP包含具有酶促活性的PIPKIalpha以及对磷酸肌醇敏感的蛋白激酶Casein Kinase Ialpha，定义了独特的磷酸肌醇核复合物，可调节选择的基因表达。定义了调节由核磷酸肌醇调节的3'端mRNA加工的分子机制，并证明了基于核磷酸肌醇的信号通路通过控制mRNA加工的3'端形成来调节mRNA水平。

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  • 作者

    Mellman, David Lorenz.;

  • 作者单位

    The University of Wisconsin - Madison.;

  • 授予单位 The University of Wisconsin - Madison.;
  • 学科 Biology Cell.
  • 学位 Ph.D.
  • 年度 2009
  • 页码 239 p.
  • 总页数 239
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

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