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Automated microfluidic sample preparation for laser scanning cytometry.

机译:自动化的微流控样品制备,用于激光扫描细胞仪。

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摘要

Laser scanning cytometry (LSC) is a slide-based method that is used clinically for Quantitative Imaging Cytometry (QIC). A "Clatch" slide, named after the inventor, which is used in conjunction with the LSC for immunophenotyping patient cell samples, has several drawbacks. The slide requires time consuming and laborious pipette steps, making the slide prone to handling errors. The Clatch slide also uses a significant amount of cell sample, limiting the number of analyses for fine needle aspirate (FNA) samples.;This thesis details an automated microfluidic system, composed of an embedded circuit, a plastic and polymer microfluidic device, and an aluminum frame, which can perform the same immunophenotyping procedures. This new system reduces the labor from 36 pipette steps to 8, it reduces the amount of cell sample from 180 muL to 56 muL, and it shortens the entire procedure from 75 minutes to 42 minutes.
机译:激光扫描细胞术(LSC)是一种基于幻灯片的方法,临床上用于定量成像细胞术(QIC)。以发明人的名字命名的“ Clatch”载玻片与LSC一起用于对患者细胞样品进行免疫表型分析,具有几个缺点。载玻片需要耗时且费力的移液步骤,使得载玻片易于处理错误。 Clatch玻片还使用了大量的细胞样品,从而限制了细针抽吸物(FNA)样品的分析数量。本论文详细介绍了一种自动微流体系统,该系统由嵌入式电路,塑料和聚合物微流体设备以及铝框,可以执行相同的免疫表型程序。这个新的系统将劳动从36个移液步骤减少到8个,将细胞样品的数量从180μL减少到56μL,并将整个过程从75分钟缩短到42分钟。

著录项

  • 作者

    Wu, Eric.;

  • 作者单位

    University of Toronto (Canada).;

  • 授予单位 University of Toronto (Canada).;
  • 学科 Engineering Industrial.;Engineering Mechanical.
  • 学位 M.Sc.
  • 年度 2010
  • 页码 1010 p.
  • 总页数 1010
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

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