• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文学位 >RNA-metal ion interactions and metal ion-induced conformational change in the spliceosomal U2-U6 snRNA complex studied by lanthanide ion luminescnece and resonance energy transfer techniques.
【24h】

RNA-metal ion interactions and metal ion-induced conformational change in the spliceosomal U2-U6 snRNA complex studied by lanthanide ion luminescnece and resonance energy transfer techniques.

机译：通过镧系离子发光和共振能量转移技术研究了剪接体U2-U6 snRNA复合物中的RNA-金属离子相互作用和金属离子诱导的构象变化。

获取原文
获取原文并翻译 | 示例
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

RNA splicing is an integral step of gene expression. In eukaryotic cells, it is carried out by a dynamic RNA-protein assembly called the spliceosome. Among its RNA components, U2 and U6 snRNAs are most conserved and form a complex with extensive base pairs. This complex is able to catalyze splicing-related reactions in the absence of proteins. Metal ions are required for the activity both in vivo and in vitro. The goal of this dissertation is to study the metal ion binding properties of the U2-U6 snRNA complex and elucidate the impact of metal ions on the complex structure. Spectroscopic informative Tb(III) is used to study RNA and metal ion binding properties. Specific metal ion binding sites and their pH-dependence on the complex are mapped out by using Tb(III) bound on RNA as FRET donors. The results not only confirm the metal ion binding sites suggested by other methods, but also reveal a new pH-dependent metal ion bindings site. Proper experimental conditions of using Tb(III) as FRET donors are suggested. By using FRET and NSET methods, the structural impact of metal ions on the U2-U6 snRNA complex is monitored. Under the conditions tested in the study, no conformational changes have been observed upon the addition of metal ions. Our results introduce the use of Tb(III) bound on RNA as FRET donor for the first time; suggest proper conditions of how to use Tb(III) as FRET donor; and show that there is no conformational changes observed by the discussed experimental designs and methods.
机译：RNA剪接是基因表达必不可少的步骤。在真核细胞中，它是通过称为剪接体的动态RNA蛋白装配来进行的。在其RNA成分中，U2和U6 snRNA最保守，并形成具有广泛碱基对的复合物。在没有蛋白质的情况下，这种复合物能够催化与剪接相关的反应。金属离子是体内和体外活性所必需的。本文的目的是研究U2-U6 snRNA复合物的金属离子结合特性，并阐明金属离子对复合物结构的影响。光谱丰富的Tb（III）用于研究RNA和金属离子结合特性。通过使用结合在RNA上的Tb（III）作为FRET供体，可以绘制出特定的金属离子结合位点及其对复合物的pH依赖性。结果不仅证实了其他方法提出的金属离子结合位点，而且揭示了新的pH依赖性金属离子结合位点。建议使用Tb（III）作为FRET供体的正确实验条件。通过使用FRET和NSET方法，可以监测金属离子对U2-U6 snRNA复合物的结构影响。在研究中测试的条件下，添加金属离子后未观察到构象变化。我们的结果首次介绍了结合在RNA上的Tb（III）作为FRET供体的用途。建议如何使用Tb（III）作为FRET供体的适当条件；并且表明通过所讨论的实验设计和方法没有观察到构象变化。

著录项

  • 作者

    Yuan, Faqing.;

  • 作者单位

    The Florida State University.;

  • 授予单位 The Florida State University.;
  • 学科 Chemistry Biochemistry.;Biophysics General.
  • 学位 Ph.D.
  • 年度 2008
  • 页码 135 p.
  • 总页数 135
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号