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首页> 外文学位 >Mapping QTL for agronomic and canning quality traits in black bean (Phaseolus vulgaris L.).
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Mapping QTL for agronomic and canning quality traits in black bean (Phaseolus vulgaris L.).

机译：为黑豆（Phaseolus vulgaris L.）的农艺和罐头品质性状绘制QTL。

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摘要

Quantitative trait loci (QTL) analysis was used to identify QTL for agronomic performance and canning quality in a recombinant inbred line (RIL) population from the cross of the black bean cultivar 'Jaguar' and the breeding line 115M. A total of 96 RILs were evaluated for seven agronomic and four canning quality traits in replicated trials at one location over four years (2004-2007) in Michigan. SSR, TRAP, SRAP, and phenotypic markers were used to create a genetic map of the population consisting of 119 loci including a locus associated with resistance to a new race of bean rust isolated in Michigan. The map consisted of 15 linkage groups spanning 460cM (38%) of the bean genome. Composite interval mapping analysis identified a total of 20 QTL for 10 traits averaged across environments, while an additional 18 QTL were identified in one or more individual environments. QTL were identified on 10 linkage groups (LG). A major QTL for seed yield was identified on LG B10. A total of 7 QTL for yield, seed size, plant height, and canned bean texture showed positive alleles from 115M. Several QTL co-localized with regions identified in previous studies while others, particularly for canning quality, were unique. Rust resistance associated with 115M was mapped to LG B4 and flanked by two TRAP markers, both at an approximate distance of 3 cM. These results support the utility of TRAP markers to tag disease resistance loci and QTL and provide a valuable source of rust resistance for future black bean cultivars adapted to Michigan.
机译：使用数量性状基因座（QTL）分析来鉴定QTL，该基因组来自黑豆品种'Jaguar'和种系115M的杂交近交系（RIL）群体的农艺性能和罐头质量。在密歇根州的一个地点进行了为期四年（2004-2007年）的重复试验，共对96个RIL进行了7个农艺和4个罐头质量性状的评估。使用SSR，TRAP，SRAP和表型标记创建了由119个基因座组成的种群遗传图谱，包括与密歇根州分离的新一类锈病相关的基因座。该图由跨越豆基因组460cM（38％）的15个连锁组组成。复合区间作图分析确定了在整个环境中平均获得的10个性状的20个QTL，而在一个或多个单独的环境中又确定了18个QTL。在10个连锁组（LG）上确定了QTL。 LG B10鉴定出主要的种子产量QTL。产量，种子大小，株高和罐头豆质地的总计7个QTL显示115M的正等位基因。一些QTL与先前研究中确定的区域共定位，而其他QTL（尤其是罐头质量）则是独特的。将与115M相关的防锈性绘制在LG B4上，并在两个TRAP标记的两侧，两者的距离均为3 cM。这些结果支持TRAP标记物标记抗病基因座和QTL的效用，并为将来适应密歇根州的黑豆品种提供了重要的抗锈源。

著录项

  • 作者

    Wright, Evan Michael.;

  • 作者单位

    Michigan State University.;

  • 授予单位 Michigan State University.;
  • 学科 Agriculture Agronomy.;Biology Genetics.;Agriculture Food Science and Technology.
  • 学位 M.S.
  • 年度 2008
  • 页码 155 p.
  • 总页数 155
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

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