• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文学位 >PCR-based identification of Legionella pneumophila, Aeromonas hydrophila, and Yersinia enterocolitica in water and sediment samples.
【24h】

PCR-based identification of Legionella pneumophila, Aeromonas hydrophila, and Yersinia enterocolitica in water and sediment samples.

机译：基于PCR的水和沉积物样品中军团菌，嗜水气单胞菌和小肠结肠炎耶尔森氏菌的鉴定。

获取原文
获取原文并翻译 | 示例
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Legionella pneumophila, Aeromonas hydrophila, and Yersinia enterocolitica are all potential human pathogens that are commonly found in the environment. These gram-negative bacilli are all notoriously difficult, expensive and time-consuming to identify. It was the purpose of this study to determine if PCR could be used as a definitive, more efficient test for these organisms. Soil and water samples were spiked with each organism to determine the sensitivity of the primers, and then water and sediment samples were tested quarterly from 15 sites along the Rio Grande. Legionella pneumophila was not detected in any environmental samples. Yersinia enterocolitica tested positive only at Arroyo Hondo, New Mexico. Aeromonas hydrophila was detected once in a microbial sample. The positive PCR control for presence of bacterial DNA, consisting of primers specific for Kingdom eubacteria, detected 81 positives in environmental samples. Overall, this study suggests that positive identification of Legionella pneumophila and Yersinia enterocolitica is more definitive with PCR than with previous culture methods. Results for Aeromonas hydrophila were insufficient for analysis. Statistical analysis of eubacteria data demonstrates that culturing is more efficient than PCR at that level.
机译：肺炎军团菌，嗜水气单胞菌和小肠结肠炎耶尔森氏菌都是在环境中常见的潜在人类病原体。众所周知，这些革兰氏阴性杆菌很难鉴定，昂贵且费时。这项研究的目的是确定PCR是否可以用作对这些生物的权威性，更有效的测试。向每种生物掺入土壤和水样品以确定引物的敏感性，然后每季度从里奥格兰德州的15个站点对水和沉积物样品进行测试。在任何环境样品中均未检出嗜肺军团菌。小肠结肠炎耶尔森菌仅在新墨西哥州的阿罗约·洪多市检测为阳性。在微生物样品中一次检测到嗜水气单胞菌。细菌DNA的阳性PCR对照（由针对王国真细菌的引物组成）在环境样品中检测到81个阳性。总的来说，这项研究表明，与以前的培养方法相比，PCR可以更准确地鉴定军团菌和小肠结肠炎耶尔森菌。嗜水气单胞菌的结果不足以进行分析。对真细菌数据的统计分析表明，在该水平上培养比PCR更为有效。

著录项

  • 作者

    Duran, Leah K.;

  • 作者单位

    Sul Ross State University.;

  • 授予单位 Sul Ross State University.;
  • 学科 Biology Microbiology.
  • 学位 M.S.
  • 年度 2008
  • 页码 45 p.
  • 总页数 45
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 微生物学;
  • 关键词

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号