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Electrical Stimulation of Human Dermal Fibroblasts and the Quantification of Collagen, Collagenase, and Elastin

机译:人皮肤成纤维细胞的电刺激以及胶原蛋白,胶原酶和弹性蛋白的定量

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摘要

Electrical stimulation of tissues has been found to have many uses in pain management, antibacterial treatment, and wound healing. In vivo, it is known to stimulate epidermal migration and increase fibroblast cell proliferation. Here the effects of electrical field (EF) stimulation on collagen, elastin, and collagenase expression in human dermal fibroblasts are studied. The cells are stimulated in bioreactor using square wave voltage pulses controlled by potentiostat for up to 24 h period. The pulse voltage (0--10V), pulse bias (0, +,-), pulse time (10--1000 ms), rest time (0.1--10 s) was varied. The effects of EF stimulation is evaluated in terms of protein expression level and changes in cell morphology. The results show that the expressions of these proteins are correlated and are doubled when EF stimulation larger than 3V and positive bias is applied. The shorter pulse time stimulates the cells more effectively, while the rest time between pulses has smaller effect.
机译:已经发现组织的电刺激在疼痛处理,抗菌治疗和伤口愈合中具有许多用途。在体内,已知刺激表皮迁移并增加成纤维细胞增殖。这里研究了电场(EF)刺激对人皮肤成纤维细胞中胶原蛋白,弹性蛋白和胶原酶表达的影响。使用恒电位仪控制的方波电压脉冲在生物反应器中刺激细胞长达24小时。改变了脉冲电压(0--10V),脉冲偏置(0,+,-),脉冲时间(10--1000 ms),静止时间(0.1--10 s)。 EF刺激的作用根据蛋白质表达水平和细胞形态变化进行评估。结果表明,当EF刺激大于3V并施加正偏压时,这些蛋白质的表达是相关的,并且是两倍。较短的脉冲时间可以更有效地刺激细胞,而脉冲之间的其余时间影响较小。

著录项

  • 作者

    Nguyen, Elise B.;

  • 作者单位

    California State University, Long Beach.;

  • 授予单位 California State University, Long Beach.;
  • 学科 Biochemistry.
  • 学位 M.S.
  • 年度 2017
  • 页码 68 p.
  • 总页数 68
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

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