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首页> 外文学位 >Respiration and energy metabolism during exercise in Callinectes sapidus, the Atlantic blue crab: Effects of the bacterial pathogen Vibrio campbellii.
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Respiration and energy metabolism during exercise in Callinectes sapidus, the Atlantic blue crab: Effects of the bacterial pathogen Vibrio campbellii.

机译：大西洋蓝蟹Callinectes sapidus在运动过程中的呼吸和能量代谢：细菌病原体坎贝尔弧菌。

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摘要

The Atlantic blue crab, Callinectes sapidus, lives in estuarine and coastal environments where exposure to disease-causing microorganisms occurs. Bacteria from the genus Vibrio are naturally abundant in marine environments and are commonly associated with the exoskeleton and within the hemolymph of blue crabs and have the potential to cause mortality in these organisms. First, the impact of the marine bacterium Vibrio campbellii on survival of blue crabs was assessed. Three replicate trials of an LD50 bacterial challenge assay were performed. We determined that V. campbellii can cause mortality in blue crabs with an average 48 h LD50 value of 6.2 x 10 5 CFU g-1 crab. Secondly, the effect of bacterial exposure on metabolism was determined following injection of V. campbellii or saline (control). Injection of V. campbellii caused a 30% reduction in resting oxygen uptake 4 h after injection, which decreased further to 42% at 24 h, accompanied only by a small increase in circulating hemolymph lactate. These data support the hypothesis that hemocyte aggregates forming around bacteria in the gills interfere with normal respiratory and circulatory function of the gills. Since blue crabs depend on the ability to effectively move around in their environment to survive, we also investigated how exposure to bacteria affects the energetic costs of 30 min of walking at 8 m min-1. Blue crabs more than doubled their aerobic and anaerobic metabolism in response to moderate walking in well-oxygenated conditions. However, exposure to V. campbellii caused a significant depression in aerobic metabolism during and after walking supplemented only by a small increase in anaerobic metabolism. Patterns of phosphagen and adenylate consumption within an active muscle were not affected. The ability of blue crabs to supply the necessary energy requirements of walking is remarkably and largely unaffected by treatment with Vibrio; however, Vibrio-injected crabs are less aerobic while doing so. This depressed metabolic condition in response to bacteria, present even during moderate activity, could be caused by a gill blockage by hemocyte aggregations in response to bacteria or may represent an internally regulated depression of metabolism.
机译：大西洋蓝蟹Callinectes sapidus生活在河口和沿海环境中，那里容易接触致病微生物。弧菌属细菌在海洋环境中自然丰富，通常与外骨骼以及蓝蟹的血淋巴相关，并有可能导致这些生物死亡。首先，评估了海洋细菌坎贝尔弧菌对蓝蟹生存的影响。进行了LD50细菌激发试验的三个重复试验。我们确定V. campbellii可以导致蓝蟹的死亡，平均48小时的LD50值为6.2 x 10 5 CFU g-1蟹。其次，在注射坎贝尔弧菌或盐水（对照）后确定细菌暴露对代谢的影响。注射坎贝氏弧菌后，注射后4 h可使静息摄氧量减少30％，在24 h时进一步下降至42％，同时循环血淋巴乳酸盐仅有少量增加。这些数据支持以下假说：g中细菌周围形成的血细胞聚集会干扰normal的正常呼吸和循环功能。由于蓝蟹依赖于在周围环境中有效移动以生存的能力，因此我们还研究了暴露于细菌如何影响在8 m min-1处行走30分钟的能量消耗。在充氧条件下适度行走时，蓝蟹的需氧和厌氧代谢增加了一倍以上。但是，暴露于坎贝氏弧菌会导致步行期间和步行后有氧代谢显着降低，而厌氧代谢仅略有增加。活跃肌肉内的磷和腺苷酸消耗模式不受影响。蓝蟹提供行走所需能量的能力显着且很大程度上不受弧菌治疗的影响；但是，注射弧菌的螃蟹在这样做时的需氧量较低。即使在中等强度的活动中，这种对细菌的抑制性代谢状况也会出现，可能是由于响应细菌的血细胞聚集导致aggregation被阻塞所致，或者可能是代谢的内部调控。

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  • 作者

    Thibodeaux, Lindy Kay.;

  • 作者单位

    College of Charleston.$bMarine Biology.;

  • 授予单位 College of Charleston.$bMarine Biology.;
  • 学科 Biology Microbiology.; Biology Animal Physiology.
  • 学位 M.S.
  • 年度 2008
  • 页码 171 p.
  • 总页数 171
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 微生物学;生理学;
  • 关键词

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