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首页> 外文学位 >Enhancement of the microbial biotransformation of (-)-trans-carveol to (R)-(-)-carvone by Rhodococcus erythropolis DCL14 in various two phase partitioning bioreactor configurations.
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Enhancement of the microbial biotransformation of (-)-trans-carveol to (R)-(-)-carvone by Rhodococcus erythropolis DCL14 in various two phase partitioning bioreactor configurations.

机译：在不同的两相分配生物反应器构型中，红球红球菌DCL14增强了（-）-反式香芹酮向（R）-（-）-香芹酮的微生物生物转化。

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Carvone is a flavor and fragrance compound that is prominent in nature and is found in the essential oils of many plants. Carvone exists as two enantiomers, (R)-(-)-carvone which has a spearmint aroma and ( S)-(+)-carvone which has a caraway aroma and can be used in a variety of applications: as a common food additive, as an antimicrobial/antifungal agent and as a potato sprout inhibitor. Carvone is currently produced by the extraction of essential oils from plants where the yield and quality of the extracted oil depends largely on successful agricultural production of dill, spearmint and caraway plants. Biotechnological production can offer a constant supply of carvone that is independent of several agricultural limitations.; In this study, it was confirmed that the substrate and product of the microbial biotransformation of trans-carveol to (R)-(-)-carvone by Rhodococcus erythropolis DCL14 can be inhibitory to the cells at high concentrations. As such, a two phase partitioning bioreactor was employed where the function of the second phase (immiscible organic solvent or solid polymer beads) was to partition the inhibitory substrate into the aqueous phase at a rate governed by the metabolic demand of the cells and uptake the inhibitory product as it accumulated in the aqueous phase. Rational selection strategies were employed when determining the appropriate organic solvent and solid polymer to be used as the second phase. The performance of the reactor was evaluated based on volumetric productivity, length of biotransformation and total volume of substrate added to the reactor. The most successful reactor configuration was one in which styrene/butadiene copolymer beads were used as a second phase in the reactor and the fermentation medium was continuously circulated through an external extraction column packed with HytrelRTM 8206 polymer beads. The volumetric productivity, length of biotransformation and total volume of substrate added to this reactor were 99 mg/L.h, 48.75 h and 35 mL, respectively whereas in the single phase benchmark reactor the performance indicators were only 31 mg/L.h, 15.25 h and 5 mL, respectively. These results clearly show the advantage of employing a partitioning bioreactor configuration for the biotechnological production of high value chemical species that exhibit cytotoxicity.
机译：香芹酮是一种香精和香精化合物，在自然界中很显着，在许多植物的精油中都可以找到。香芹酮以两种对映异构体的形式存在，（R）-（-）-香芹酮具有留兰香的香气，（S）-（+）-香芹酮具有香芹香的香气，可用于多种应用：作为常见食品添加剂，作为抗微生物/抗真菌剂和马铃薯芽苗抑制剂。目前，香芹酮是通过从植物中提取香精油来生产的，这些植物中的香精油的产量和质量在很大程度上取决于莳萝，留兰香和香菜植物的成功农业生产。生物技术生产可以提供恒定的香芹酮供应，而不受几个农业方面的限制。在该研究中，证实了红血球红球菌DCL14将反式香芹酚微生物生物转化为（R）-（-）-香芹酮的底物和产物可以在高浓度下抑制细胞。因此，使用了两相分配生物反应器，其中第二相（不混溶的有机溶剂或固体聚合物珠粒）的功能是将抑制性底物以细胞代谢需要控制的速率分配到水相中并吸收抑制产物，因为它在水相中积累。当确定合适的有机溶剂和固体聚合物用作第二相时，采用合理的选择策略。基于体积生产率，生物转化的长度和添加到反应器中的底物的总体积来评估反应器的性能。最成功的反应器配置是将苯乙烯/丁二烯共聚物珠用作反应器中的第二相，并使发酵培养基连续循环通过装有HytrelRTM 8206聚合物珠的外部萃取柱。加入该反应器的体积生产率，生物转化的长度和底物的总体积分别为99 mg / Lh，48.75 h和35 mL，而在单相基准反应器中，性能指标仅为31 mg / Lh，15.25 h和5 mL，分别。这些结果清楚地表明了采用分区生物反应器配置用于生物技术生产具有细胞毒性的高价值化学物种的优势。

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