目的：比较复合环孢菌素同种异体骨(cyclosporine-impregnated allograft bone，CAB)与冻干同种异体骨(freeze-dried allograft bone，FDAB)移植后的免疫学反应。方法60只新西兰大白兔随机分为材料提供组、实验组和对照组，每组20只。取材料提供组双侧髂骨制备CAB及FDAB。实验组、对照组动物制备右侧桡骨中段10 mm的骨与骨膜缺损，实验组植入CAB修复，对照组植入FDAB修复。术后2周、4周、8周、12周每组各取5只动物，进行全血及移植部位骨组织IL-2、TNF-α浓度测定。结果术后2周、4周、8周、12周血中IL-2、TNF-α浓度两组之间无差异。术后2周、4周移植部位骨组织中IL-2、TNF-α浓度实验组低于对照组(P值分别为0.000、0.015)，术后8周、12周移植部位骨组织中IL-2、TNF-α浓度两组之间差异均无统计学意义(P＞0.05)。结论 CAB移植后不会引起全身免疫反应，其引发的移植局部免疫排斥反应弱于FDAB。%Objective To compare the immunoreaction of cyclosporine-impregnated allograft bone(CAB) and freeze-dried allograft bone(FDAB). Methods Sixty New Zealand white rabbits were divided into bone donor group, experimental group,and control group (20 in each group). CAB and FDAB were prepared with the bilateral iliac bone from donor group. A 10 mm long bone defect in the right radius middle segment in experimental group and control group was repaired with CAB and FDAB, respectively. Serum and bone at the defect site IL-2 and TNF-αlevels were measured in 5 animals of each group at weeks 2, 4, 8 and 12 after operation. Results There were no significant difference in serum IL-2 and TNF-αlevels between two groups at weeks 2, 4, 8, 12 after operation. The bone at the defect site IL-2 and TNF-αlevels were significantly lower in experimental group than in control group at 2 and 4 weeks after operation(P<0.05). However,no significant difference was found in bone at the defect site IL-2 and TNF-αlevels between the two groups at 8 and 12 weeks after operation(P>0.05). Conclusion CAB does not result in general immunoreaction. Its local immune rejection is weaker than FDAB.
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