Objective: To investigate changes of phosphorylated p38MAPK and ANK expression after the degeneration of lumbar endplate chondrocytes.Methods: Endplate chondrocytes were isolated by enzyme digestion and cultured in vitro to develop the natural degeneration model of lumbar endplate chondrocytes, which was identified with toluidine blue staining and immunohistostaining.Western-blotting was used to detect the expression of p38MAPK and ANK of the primary and the fourth generation, and the mRNA expression of ANK gene of the primary and the fourth generation was detected by RT-PCR.Results: The phosphorylated p38MAPK was highly expressed in the endplate chondrocytes of the fourth generation, whereas ANK was down-regulated significantly, as compared with the primary cultures( P ＜ 0.01; P ＜ 0.05 ).Conclusion: The degeneration of lumbar endplate chondrocytes with increased p38MAPK expression but with weakened ANK suggests that prevention of degeneration of the endplate cartilage can be achieved through regulating the expression of p38MAPK and ANK.%目的:探讨终板软骨细胞退变后磷酸化p38MAPK和ANK的表达变化.方法:取大鼠腰椎终板软骨细胞,酶消化法及自然传代法分离培养大鼠终板细胞,建立终板软骨细胞体外自然退变模型.采取甲苯胺蓝染色及免疫组织化学染色法,对该模型进行鉴定.以western-blot检测原代及第四代磷酸化p38MAPK及ANK的表达;RT-PCR检测原代及第四代Ank基因mRNA表达.结果:与原代相比第四代终板软骨细胞磷酸化p38MAPK表达量明显增加(P<0.01);ANK表达量明显下调(P<0.05).结论:终板软骨细胞的退变伴随p38MAPK表达增强和ANK蛋白的表达减弱,两者与终板软骨细胞的退变存在明显关联,提示通过调控p38MAPK和ANK的表达可能影响终板软骨细胞的退变,可能有助于阻止终板软骨的退变.
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