目的探讨前列腺素DP受体(DPR)缺乏对小鼠脑内组胺含量的影响.方法在乙醚麻醉下,分别于明时(8:AM)及暗时(8:00PM)断头处死野生型及DPR基因敲除型小鼠,迅速取出脑组织并分离出皮层、纹状体、海马、下丘脑、丘脑、中脑及脑干等脑区,制备匀浆并用HPLC荧光检测法测量其组胺含量.结果明时处死,DPR基因敲除型小鼠皮层、下丘脑及丘脑中的组胺含量明显低于野生型小鼠;暗时处死DPR小鼠,仅丘脑中组胺含量显著降低.结论DPR基因敲除型小鼠脑中组胺能神经活性显著降低,这一变化可能是该种小鼠尚能维持正常睡眠的代偿机制之一.%Objective To explore the effect of DP receptor (DPR) deficiency on brain histamine contents in mice. Methods Wild type (WT) and DPR knockout (KO) mice were killed by decapitation under anesthesia by diethyl ether at either light on phase (8:00 AM) or light off phase (8:00 PM). The brain tissues were quickly removed out and divided on ice into seven regions: the cortex, striatum, hippocampus, hypothalamus,thalamus, midbrain and medulla (brainstem). These brain regions were homogenized and histamine contents in the homogenates were determined by HPLC- fluorometry. Results At light on phase, histamine contents in hypothalamus, thalamus and cortex were significantly lower in DPR-KO mice than in WT mice (P = 0. 0384,0.0455 and 0. 0179, respectively). However, at light off phase the difference was only observed in the thalamus. Conclusion The activity of the histaminergic system decreased and the decrease might be one of the compensatory mechanisms for maintenance of the normal sleep in the null mice lacking DPR.
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