CorA/MRS2/MGT-型镁离子转运蛋白在维持植物镁离子平衡中具有重要作用.为探讨一个表达受缺镁胁迫诱导的玉米MRS2/MGT-型镁离子转运蛋白基因ZmMGT10在缺镁胁迫中的功能,构建了ZmMGT10的过表达载体并遗传转化拟南芥,获得了转基因拟南芥株系.同野生型拟南芥植株相比,过表达ZmMGT10增加了低镁胁迫生长下转基因植株的生物量、根长和叶绿素浓度.进一步研究表明,在低镁生长条件下,转基因植株的根和叶中积累的镁离子含量均明显高于野生型植株.此外,在低镁生长条件下,转基因植株根对镁离子的吸收能力明显强于野生型植株.结果表明,过表达ZmMGT10基因可以增强转基因拟南芥植株对低镁胁迫的抵抗.%CorA/MRS2/MGT-type magnesium ion transporters play an important role in maintaining magnesi-um homeostasis in plants. In order to investigate the function of ZmMGT10 gene which is a maize CorA/MRS2/MGT-type magnesium ion transporter and expression is induced by magnesium deficiency,we constructed the over-expression vector of ZmMGT10 and transgenic Arabidopsis plants were obtained. Compared with wild-type plants, transgenic Arabidopsis plants grew more vigorously than wild-type plants under low Mg conditions,exhibited by lon-ger root length,higher plant fresh weight and chlorophyll content. Further analysis indicated that the roots and shoots of transgenic plants had higher magnesium accumulation than wild-type plants under low magnesium condition. Fur-thermore,the roots of transgenic plants had enhanced magnesium uptake ability than wild-type plants. The results suggested that overexpression ZmMGT10 in Arabidopsis could enhance the resistance ability of transgenic plants to magnesium deficiency stress.
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