Solanum torvum,a wild species of eggplant,is highly resistant to Verticillium wilt.Therefore,it is the ideal material for studying the mechanism of resistance to Verticillium wilt in eggplant.Late embryogenesis abundant proteins (LEA proteins) are a group of stress-responsive proteins in higher plants that are induced by environmental stress.In this study,a up-regulated EST while infected by Verticillium wilt was used as a querying probe to blast the GenBank database.Based on the assembled homologous cDNA sequences ,a 490 bp cDNA was amplified and cloned by RT-PCR,designated StLEA1.The ORF of StLEA1 is 291 bp,coding a protein with 96 animo acids.The molecular weight of coding protein is 10.748 kDa and isoelectric point is 9.913.StLEA1 protein is a soluble protein with mul-tiple phosphorylation sites,conserve domain in Lea5,but without signal peptides .Quantitative RT-PCR analysis re-vealed that StLEA1 was up-regulated in roots while Solanum torvum was infected by Verticillium wilt.The result pro-vides the material for studying the resistance mechanism of wild eggplant to Verticillium wilt.% 野生茄托鲁巴姆高抗黄萎病，是研究茄子黄萎病抗性的理想试材。胚胎发育晚期丰富蛋白（LEA 蛋白）是植物在逆境条件下生成的一类应激蛋白。研究以1个受黄萎病菌诱导的托鲁巴姆 EST 为种子序列，结合电子克隆及RT－PCR 验证的策略，获得了 LEA 蛋白基因的全长 cDNA 序列，命名为 StLEA1。该基因的完整开放阅读框为291 bp，编码96个氨基酸，编码蛋白的分子量为10．748 kDa，等电点为9.913。经序列分析，StLEA1为水溶性蛋白，不存在信号肽，具有 LEA5家族的典型结构域和保守序列，预测含有多个磷酸化位点。表达分析表明，托鲁巴姆受黄萎病菌侵染后，StLEA1在根系中上调表达。为探讨野生茄托鲁巴姆抗黄萎病分子机制提供了素材。
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