首页> 中文期刊> 《华北农学报》 >氟磺胺草醚降解菌 BX3的分离、鉴定与降解特性研究

氟磺胺草醚降解菌 BX3的分离、鉴定与降解特性研究

         

摘要

In order to decrease the amount of fomesafen in soil ,a high efficiency degradation strain BX3 was screened from the activated sludges by enrichment culture .The strain was preliminary identified as Stenotroph-omonas acidaminiphila according to its colony morphology ,physiological and biochemical characteristics and 16S rDNA gene sequence analysis.Meanwhile,the growth characteristics and degradation characteristics of strain BX 3 were preliminary studied.The optimum conditions of fomesafen degradation were as follows :concentration of fome-safen culture solution of 100 mg/L,incubation temperature of 30 ℃ and pH value of 6 -7.The result shows that strain BX3 had strong fomesafen degradation capacity under the optimum conditions .The removal efficiency of fome-safen could reach more than 80% after incubation of 5 days.The strain BX3 was a suitable candidate in bioremedi-ation of fomesafen-contaminated soil.%  为了减轻氟磺胺草醚对土壤的污染问题,利用富集培养技术从山东某农药厂活性污泥中筛选到1株具有高效降解氟磺胺草醚能力的细菌,命名为 BX3。通过菌落形态、生理生化特征和16S rDNA 基因序列分析,初步鉴定菌株 BX3为微嗜酸寡氧单胞菌。同时对菌株 BX3的生长特性及降解特性进行了初步研究。结果表明,该菌株降解氟磺胺草醚的最适条件为:氟磺胺草醚质量浓度为100 mg/L,培养温度为30℃,pH 值6~7。在最适条件下,菌株 BX3具有较强的降解氟磺胺草醚的能力,培养5 d 后对氟磺胺草醚的降解率达到80%以上。可为氟磺胺草醚污染土壤的生物修复提供适宜的菌种资源。

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