According to the amino acid sequence of the sweet protein gene thaumatin and plant biased codons, the whole gene sequence primer of thaumatin I was designed. The whole gene of thaumatin I was artificially synthesized by overlap extension PCR method , and then it was cloned to pMD9 - T. The sequence analysis result showed that the synthesized DNA sequence was entirely accordant with the original designed sequence. The high - efficient plant expressive vector pRIlOl - thaumatin was finally constructed by u-sing the expressive vector pRl10l -ON.%根据thaumatin氨基酸序列及植物蛋白质表达密码子的偏爱性,设计了thaumatinⅠ的全基因序列引物,采用重叠延伸PCR法,人工合成了thaumatinⅠ全长基因,克隆至载体MD19-T上,序列测序表明,克隆的DNA序列与原设计序列完全相同,利用表达载体pRI101-ON,构建了高效植物表达载体RI101-thaumatin.
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