首页> 中文期刊> 《上海农业学报》 >转基因抗虫水稻秀水134-Bt和非转基因亲本的稻米主要品质性状与淀粉链长分布比较

转基因抗虫水稻秀水134-Bt和非转基因亲本的稻米主要品质性状与淀粉链长分布比较

         

摘要

Xiushui 134-Bt was a transgenic homozygous line developed through inserting the insect-resistant gene cry1Ac1 into keng rice'Xiushui 134'by agrobacterium mediated transformation.The rice quality characters,starch physicochemical properties and amylopectin chain length distribution were comparatively analyzed between it and its non-transgenic parent'Xiushui 134'.It was found that Xiushui 134-Bt and'Xiushui 134'was very similar and basically the same in terms of apparent quality,milling quality,amylose content,gel consistency,starch viscosity and amylopectin chain length distribution,indicating that the insertion of T-DNA did not significantly change the parent's main quality characters.%秀水134-Bt是以‘秀水134’为受体材料,通过根癌农杆菌介导的遗传转化方法导入抗虫基因cry1Ac1获得的转基因纯合品系,本研究对抗虫基因水稻秀水134-Bt与其原亲本‘秀水134’稻米的品质性状、理化特性及支链淀粉链长分布进行了比较分析.结果发现:转基因水稻秀水134-Bt与非转基因亲本‘秀水134’非常相似,稻米主要品质性状包括外观品质、碾磨品质、直链淀粉含量、胶稠度、淀粉黏滞性及支链淀粉的链长分布等食用品质方面基本相同,说明在水稻遗传转化操作过程中,T-DNA插入并未显著改变原亲本稻米的主要品质特性.

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