One porcine pseudorabies virus was isolated from epidemiological investigation,which was named PRV C strain,then the specificity of the strain was studied.The special prime during the region gI and gE was designed to check the strain by PCR,it showed that objective band of Min A strain lied at about 5 000 bp,but the strain lied at about 1 500 bp gene sequencing showed that the sequencing was 1 538 bp.The gene deletion of the strain was during 989—4 468,about 3 570 bp.The deletion consisted of the main virulence(gE),the muta-tions happened at the site of 873,4 665,4 666,4 950.The homology of the strain and Bartha-K61 was during 95.0%—96.0%,so it was different from the Bartha-K61.The neutralization test was done on neutralization of the virus antibody by the 14 strains PRV isolated from some provinces in China and Min A strain.The neutraliza-tion indexes were 708—6 761,the strain could neutralize all the strains PRV,which indicated that it could be the candidate vaccine strain.%在流行病学调查中分离到的1株伪狂犬病毒，命名为猪伪狂犬病毒 C 株，并对其进行鉴定、纯化。在 gE 和 gI 基因段上设计特异性引物进行 PCR 检测，闽 A 株在5000 bp 处出现目的条带，该毒株在1500 bp 附近出现目的条带，对目的条带测序，扩增产物大小为1538 bp，在第989—4468位基因缺失，缺失片段大小为3570 bp，该缺失片段包含了伪狂犬病病毒的主要毒力基因（gE 基因），除此缺失片段外，分别在第873、4665、4666、4950位发生了碱基突变，同猪伪狂犬病病毒 Bartha K61同源性为95.0％—96.0％，与 PRV Bartha K61不是同一个毒株。用该毒株对部分省市分离到的伪狂犬毒株和标准毒株闽 A 株进行中和试验，中和指数为708—6761，该毒株可完全中和临床分离的伪狂犬病毒株，故可用作候选疫苗毒株。
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